国产多孔玻璃碳的细胞相容性及犬体内植入的实验研究  

Study on cytocompatibility and animal implantation test of domestic reticulated vitreous carbon

作　　者：尉晓蔚[1] 赵德伟[1] 张魁伟[1] 王建川[1] 王本杰[1] 王威[1] 

机构地区：[1]大连大学附属中山医院骨科医学研究中心,辽宁省大连市116001

出　　处：《中华显微外科杂志》2016年第4期354-358,共5页Chinese Journal of Microsurgery

基　　金：国家自然科学基金项目（81371942）;大连市科技局项目（2013J21DW004）

摘　　要：目的探讨同产多孔玻璃碳作为骨组织工程载体的支架材料的可能性。方法自2013年6月-2014年8月．原代培养的犬骨髓基质干细胞与多孔玻璃碳体外联合培养后，观察细胞的形态及其增殖情况。使用单因素方差分析检测多孔玻璃碳的细胞毒性。雄性实验犬3只，在其右后肢建立大转子骨缺损模型，将多孔玻璃碳分别植入犬大转子骨缺损位点及腹股沟，每只犬的左后肢大转子设为对照组。12周后，观察多孔玻璃碳在犬体内的生物相容性。结果MTT结果显示骨髓基质干细胞与多孔玻璃碳分别联合培养1、3、5、7d后，其增殖情况与体外培养差异无统计学意义（P〉0．05）。倒置荧光显微镜及扫描电镜发现骨髓基质干细胞黏附于材料孔隙之间，细胞充分伸展，向四周伸出伪足，分泌的细胞外基质包绕于细胞周围。多孔玻璃碳植入腹股沟12周后，材料周同和内部新生出了结缔组织，并且没有发现局部肿瘤和炎症反应。多孔玻璃碳植入大转子骨缺损位点12周后，与宿主骨的骨小梁产生稳定连接，材料与缺损区周围的松质骨结合紧密。结论目产多孔玻璃碳支架无细胞毒性，在体外能促进骨髓基质干细胞的黏附与增殖，在体内具有良好的骨诱导特性。Objective To investigate the possibility of the domestic reticulated vitreous carbon as a kind of scaffold material for hone tissue engineering, the biocompatibility of domestic reticulated vitreous carbon was first successfully tested with bone manow stromal cells （BMSCs） in vitro and for bone tissue repair in vivo. Methods From June, 2013 to August, 2014, the morphology and proliferation of BMSCs co-cultrued with scaffold material in vitro was measured. Differences of measurement were compared with single factor analysis of variance to detect the cytotoxieity of reticulated vitreous carbon. In vivo reticulated vitreous carbon were implanted into the bone defect site and the groin. After 12 weeks, the bioeompatibility of reticulated vitreous carbon was observed. Results MTT results showed that after 7d co-culture, the survival and proliferation of BMSCs had not been significantly inhibited （P 〉 0.05）. Inverted fluorescence microscope and scanning electron microscope found that newly developed threedimensional domestic reticulated vitreous carbon could promote adhesion, aggregation and proliferation of BMSCs in vitro. Studies in vivo demonstrate that implanted reticulated vitreous carbon with a high porosity and host bone may produce a stable connection and integration. Conclusion Non-eytotoxie domestic reticulated vitreous carbon can promote the adhesion and proliferation of bone marrow mesenehymal stem cells in vitro and has good bone induction properties in vivo.

关 键 词：多孔玻璃碳 骨髓基质干细胞 生物相容性 骨缺损 犬 

分 类 号：R318.08[医药卫生—生物医学工程]