米非司酮对前脂肪细胞的分化及核因子κB激活的影响  

Effect of RU486 on pre-adipocytes differentiation and NF-κB activation

作　　者：王毅飞[1] 伊娜 吴琳英[1] 黄晓淳[1] 梁思虹 

机构地区：[1]广州医科大学附属第一医院内分泌科,510120 [2]广州市中医院内分泌科,510130

出　　处：《实用医学杂志》2016年第15期2434-2437,共4页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2013B021800195);广州医科大学附属第一医院立项课题(编号:201210-gyfyy)

摘　　要：目的：探讨米非司酮（RU486）对前脂肪细胞分化及核因子κB（NF-κB）激活的作用。方法：形态观察3T3-L1细胞分化过程,不同RU486浓度的药物（0.1～10 mmol/L）处理细胞48 h,于分化的第9天油红O染色测定甘油三酯相对含量,实时荧光定量PCR法检测PPARγ2、C/EBPa、脂蛋白脂酶（LPL）和脂肪酸合成酶（FAS）的m RNA表达。免疫印迹检测IкBα蛋白水平,免疫荧光观察NF-κB蛋白核移位。结果：甘油三酯相对含量随药物浓度的增加而降低,与对照组比,从0.5μmol/L RU486处理开始甘油三酯相对含量显著性降低（P〈0.05或P〈0.01）。与对照组比,5μmol/L RU486组的PPARγ2、C/EBPa、LPL和FAS m RNA表达均显著性降低（P〈0.01）,IκBα蛋白含量降低（P〈0.01）,NF-κB由细胞浆到细胞核的核移位。结论：RU486可下调IκBα蛋白水平,激活NF-κB核移位,下调PPARγ2、C/EBPa、LPL和FAS的m RNA表达,抑制前脂肪细胞分化。Objective To investigate the roles of RU486 inhibiting 3T3-L1 pre-adipocytes differentiation and regulating NF-κB activation. Methods Cells were treated with RU486 with concentrations of 0.1 ～ 10μmol / L for 48 h, then the relative contents of triglyceride were analyzed by Oil-Red O staining assay on 9thday during adipogenesis. The m RNA expressions of PPARγ2,C / EBPa, LPL and FAS were further measured by Realtime PCR. IκBα protein level was detected by Western bolt and nuclear translocation of NF-κB was observed by immunofluorescence assay. Results The relative contents of triglyceride decreased with the increasing of RU486 concentration. Compared with the control, the relative contents of triglyceride in RU486-treatment groups from0.5 μmol / L were significantly decreased（P〈0.05 or P〈0.01）. Compared with the control, PPARγ2, C /EBPa, LPL and FAS m RNA expression and IκBα protein level were significantly decreased（P〈0.01） and NF-κB nuclear translocated from cytoplasm to nucleus in Group 5 mmol / L RU486. Conclusions RU486 could down regulate IκBα protein level, activate NF-κB nuclear translocation, then down regulate PPARγ2, C / EBPa,LPL and FAS m RNA expression and inhibit adipocytes differentiation.

关 键 词：米非司酮 NF-ΚB 脂肪细胞分化 3T3-L1细胞 

分 类 号：R96[医药卫生—药理学]