KLF7联合脂肪源性干细胞对小鼠坐骨神经缺损后轴突再生的影响  

Effects of KLF7 and ADSC transplantation on expression of BDNF and its receptor TrkB after sciatic nerve injury in mice

作　　者：李文媛[1] 王莹[1] 李智刚[2] 闫哲[1] 杨春壮[1] 李凯军[1] 赵微[1] 

机构地区：[1]牡丹江医学院解剖学教研室,黑龙江牡丹江157011 [2]牡丹江医学院红旗医院普外科,黑龙江牡丹江157011

出　　处：《中风与神经疾病杂志》2016年第7期580-583,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金(No.81371362);黑龙江省自然科学基金项目(No.H201491);黑龙江省卫生计生委立项科研课题(No.2014-211);牡丹江医学院科学技术研究项目(No.2s201310)

摘　　要：目的探讨核转录因子KLF7与脂肪源性干细胞(ADSC)联合应用对小鼠脱细胞同种异体神经支架(ANA)移植坐骨神经缺损后轴突再生和功能恢复的影响。方法成年C57BL/6小鼠随机分为脱细胞神经支架(ANA)组、ADSC组和KLF7+ADSC组,每组10只。坐骨神经功能指数(SFI)和电生理方法检测神经运动功能的恢复,Western bolt检测神经移植体内KLF7、Trk A和Trk B的蛋白表达。NF200免疫荧光法检测神经支架中轴突再生,并示踪PKH26标记的移植ADSC。结果与ADSC组比较,KLF7+ADSC组神经移植体内KLF7、Trk A和Trk B蛋白表达明显增高,NF200表达增强,PKH-26标记的ADSC数量显著增高(P<0.05)。与ANA组比较,ADSC组和KLF7+ADSC组电生理波幅增高、神经传导速度增快、延迟期缩短,SFI功能指数增高,其中KLF7+ADSC组神经恢复作用显著优于ADSC组(P<0.05)。结论 KLF7联合ADSC移植对小鼠ANA修复坐骨神经缺损后轴突再生和功能恢复的作用优于ADSC组,其机制可能与KLF7高表达促进神经移植体内Trk A和Trk B表达,进而促进移植的ADSC存活有关。Objective To study the effect of KLF7 and adipose-derived stem cell（ADSC） transplantation on expression of axonal regeneration and function recovery after acellular nerve allograft（ANA） repair of the sciatic nerve gap in mice.Methods Adult C57 BL/6 mice were randomly divided into ANA group,ADSC group and KLF7 ＋ ADSC group.The sciatic functions index（SFI） and electrophysiology were used to evaluate functional recovery.KLF7,Trk A and Trk B protein were evaluated by Western bolt,the protein expression of NF200 and the fluorescence signal of ADSC labeled with PKH-26 in the nerve graft was observed by using immunofluorescence.Results Compared with ANA group,the expression of KLF7,Trk A,Trk B,NF200 and PKH-26 labeled ADSC in KLF7 ＋ ADSC group were increased（P〈0.05）.Compared with ANA group,nerve conduction velocity,wave amplitude and the SFI score were significantly increased in ADSC group and KLF7 ＋ ADSC group（P〈0.05）,however,incubation period were significantly decreased in two treatment groups（P〈0.05）,and the effect of KLF7 ＋ ADSC group was more powerful than ADSC group（P〈0.05）.Conclusion The combined KLF7 and ADSC transplantation treatment promoted axon regeneration and function recovery much significantly than ADSC treatment,which may be related to KLF7 upregulating Trk A and Trk B expression in the ANA,and promoting ADSC survival.

关 键 词：KLF7 脂肪源性干细胞 脱细胞异体神经 TRKA TRKB 

分 类 号：R496[医药卫生—康复医学]