不同海拔牦牛颈动脉体Ⅰ型细胞线粒体和EDCV的体视学  

Stereologic study on mitochondria and EDCV of carotid body typeⅠcells of yaks at different altitudes

作　　者：张寿[1] 雷乃虎 常兰[1] 严作云 沈明华[1] 尚果玛 东珠才让 

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海省都兰县畜牧兽医站,青海都兰816100

出　　处：《中国兽医学报》2016年第8期1371-1375,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31360590);青海省科技应用基础资助项目(2014-ZJ-711)

摘　　要：颈动脉体(carotid body,CB)是第一个被发现的缺氧感知器官,当血液PaO2降低时,迅速引起呼吸加深、加快等反应。为探讨牦牛不同低氧程度下CB的适应特征,应用透射电子显微镜技术和体视学方法测量了海拔4 600,3 800和2 800m牦牛颈动脉体Ⅰ型细胞线粒体、电子致密核心囊泡体(electron dense-cored vesicles,EDCV)的体密度(Vv,单位体积Ⅰ型细胞浆中线粒体、EDCV的体积百分比)、面密度(Sv,单位体积Ⅰ型细胞浆中线粒体、EDCV外膜的表面积)、面数密度(NA,单位面积中线粒体、EDCV数目)、比表面(δ,线粒体、EDCV表面积与其自身体积之比)。结果表明,牦牛颈动脉体Ⅰ型细胞浆内线粒体的Vv值随着海拔的升高呈增加趋势,但3组间差异不显著(P>0.05);Sv、NA、δ值随着海拔从2 800m升到3 800m先减小,从3 800m到4 600m而增加,3组间差异均不显著(P>0.05)。牦牛颈动脉体Ⅰ型细胞浆内EDCV的Vv、Sv值随海拔升高呈上升趋势,但3组间差异均不显著(P>0.05);δ值随海拔升高呈下降趋势,3组间差异也不显著(P>0.05)。Carotid body is the first discovered oxygen sensory organs and can cause deep and rapid breathing when low blood PaO2. To study the adaptive characteristics of CB in the different degree of hypoxia,the volume density （Vv）,surface density （Sv）,numerical density on area （NA） and specific surface （3） of mitochondria and EDCV of CB type I cells of yaks at altitudes of 4 600 m, 3 800 m and 2 800 m were obtained by transmission electron microscopy and stereology. The re- sults showed that the Vv of mitochondria was a rise with increasing altitude,there was no signifi： cant difference among 3 altitudes,respectively （P〉0.05） ;the SV,NA ,3 values of mitochondria de- creased with rising altitude from 2 800 m to 3 800 re,and increased with rising altitude from 3 800 m to 4 600 m,there were no significant differences among 3 groups,respectively （P〉0. 05） ;the Vv, Sv values of EDCV were a rise with increasing altitude, there were not significant difference among altitudes（P〉0. 05）,respectively. The 3 value of EDCV with increasing altitude was a decline, there were no significant differences among 3 groups,respectively （P〉0. 05）.

关 键 词：线粒体 电子致密核心囊泡 颈动脉体 牦牛 体视学 不同海拔 

分 类 号：S814[农业科学—畜牧学]