小鼠自由饮10%酒精3个月对肝脏的影响  

Effects of 3-month Freely Drink with 10% Alcohol on Mouse Livers

作　　者：徐燕[1,2] 曾勇 陈育尧[2] 

机构地区：[1]赣南医学院 [2]南方医科大学中医药学院,广东广州510515 [3]赣州市食品药品监督管理局,江西赣州341000

出　　处：《赣南医学院学报》2016年第3期362-363,366,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:探讨成年小鼠自由饮10%酒精,连续3个月后对肝脏、部分肝功能及血脂的影响。方法:将小鼠分为饮酒组和正常对照组,雌雄各半,每组10只。饮酒组自由饮10%酒精3个月,正常对照组自由饮自来水。各组小鼠自由进食,3个月后空腹摘除眼球采血,测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及甘油三酯(TG)、总胆固醇(TC)含量;取小鼠肝称重,计算肝指数。并取肝左叶用10%中性福尔马林固定,常规脱水,石蜡包埋,HE染色,光镜下检查。结果:与正常对照组比较,饮酒组组小鼠血清AST活性明显升高(P<0.01);血清TG含量也有一定程度的升高(P=0.076);饮酒组小鼠肝指数明显增加(P<0.01),肝脏明显脂肪变及坏死(P<0.01、P<0.05)。结论:小鼠自由饮10%酒精3个月,会造成肝细胞肿胀、脂肪变及坏死。Objective： To study the effect of 3-month freely drink with 10% alcohol on partial liver function and blood lipid levels of adult mice. Methods： The mice were divided into drinking group and normal control group with equal number of male and female（ 10 mice in each group）. The mice in drinking group freely drank 10% alcohol for 3 months,and the mice in normal control group freely drank tap water for 3 months. The mice of every group freely ate food. After 3months,we took blood samples from mouse eyeballs in fasting conditions,and tested the activity of the serum alanine aminotransferase（ ALT）,aspartate transaminase（ AST） and the levels of the triglyceride（ TG） and total cholesterol（ TC）; We then weighted the livers and calculated the liver index. The left lobe of liver were fixed in 10% neutral formalin,dehydrated,embedded in paraffin,HE stained and inspected under light microscope. Results： In comparison to control group,serum AST activity of mice in drinking group significantly increased（ P〈0. 01）; The serum TG levels also elevated to some extent（ P = 0. 076）; The liver index were raised remarkably（ P〈0. 01） and the livers of mice in drinking group showed more fatness and necrosis than controls（ P〈0. 01,P〈0. 05）. Conclusion： Three-month freely drink with10% alcohol in mice led to swelling of the livers,fat metamorphosis,and necrosis.

关 键 词：小鼠 酒精 血脂 转氨酶 病理组织检查 

分 类 号：R961[医药卫生—药理学]