铝胁迫下质膜H^+-ATPase对水稻硝态氮吸收的影响  

Effects of PM H^+-ATPase on the nitrate uptake of rice under aluminum stress

作　　者：周小华[1,2] 徐慧妮[1] 谷照虎 陈丽梅[1] 李昆志[1] 

机构地区：[1]昆明理工大学生命科学与技术学院生物工程技术研究中心,昆明650500 [2]云南商务信息工程学校,昆明650204

出　　处：《植物生理学报》2016年第7期1011-1018,共8页Plant Physiology Journal

基　　金：国家自然科学基金(31260297和31560351);云南省九湖水专项治理项目(KKK0201126044)~~

摘　　要：为了研究铝胁迫下水稻质膜H^+-ATPase对硝态氮吸收影响,以‘滇优35号’(粳稻)为试验材料,采用溶液培养法研究50μmol·L^(-1) Al^(3+)胁迫下添加质膜H^+-ATPase的激活剂壳梭孢素(FC)和抑制剂腺苷-5’-单磷酸(AMP)对质膜H^+-ATPase水解活性和H^+-泵活性,质膜H^+-ATPase和14-3-3蛋白的相互作用对水稻NO_3^--N吸收的影响。结果表明,AMP处理使铝胁迫下水稻质膜H^+-ATPase水解活性、H^+-泵的活性,质膜H^+-ATPase和14-3-3蛋白的相互作用下降,H_2O_2和MDA含量增加,从而导致水稻NO_3^--N吸收降低。FC处理可使铝胁迫下水稻质膜H^+-ATPase水解活性、H^+-泵的活性以及质膜H^+-ATPase和14-3-3蛋白的相互作用增加,H_2O_2和MDA含量下降,从而使水稻NO_3^--N吸收增加。这些研究结果表明铝胁迫通过影响质膜H^+-ATPase与14-3-3蛋白互作来影响质膜H^+-ATPase水解活性和氢泵活性以及H_2O_2和MDA含量,进而影响对硝态氮的吸收。The aim of the experiment was to study the effects of the plasma membrane（PM） H~＋-ATPase on the nitrate（NO_3^-- N） uptake of rice under aluminum（Al） stress. A hydroponic experiment was conducted with the rice hybrid ‘Dian superior 35＇（Hybrid, Japonica） to study the effects of activator Fusicoccin（FC） and inhibitor Adeosine- 5＇-Monophosphate（AMP） of PM H~＋-ATPase on the activities of PM H~＋-ATPase hydrolysis and H~＋-pump and the interaction of PM H~＋- ATPase and 14-3-3 protein on the uptake of NO_3^--N in root of the rice under 50 μmol·L^（-1） Al stress. The results showed that Al combing with AMP treatment could decrease the activities of PM H~＋-ATPase hydrolytic and H~＋-pump and the interaction of PM H~＋-ATPase and 14-3-3 protein and enhanced the contents of H_2O_2 and malondialdehyde（MDA）, which decreased the NO_3^--N uptake; Al combining with FC treatment could increase the activities of both PM H~＋-ATPase hydrolysis and H~＋-pump, decreased the contents of H_2O_2 and MDA, which promoted the NO_3^--N uptake of rice compared with single Al treatment. These results indicated that Al stress affects the activities of PM H~＋-ATPase hydrolytic and H~＋-pump as well as the contents of H_2O_2 and MDA by influencing the interaction of PM H~＋-ATPase and 14-3-3 protein, thereby affecting the absorption of nitrate.

关 键 词：铝胁迫 水稻 质膜H^+-ATP酶 硝态氮 FC AMP 

分 类 号：S511[农业科学—作物学] Q945.78[生物学—植物学]