叶面喷施钼肥对草莓幼苗氮代谢关键酶活性与^(15)N吸收、分配及利用的影响  

Effect of sodium molybdate foliar sprays on key enzymes activities of nitrogen metabolism and ^(15)N absorption, distribution and utilization of strawberry seedlings

作　　者：刘利[1] 张蕊[1] 杨超[1] 李玲[1] 高东升[1] 

机构地区：[1]作物生物学国家重点实验室,山东果蔬优质高效生产协同创新中心,山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《植物生理学报》2016年第7期1035-1044,共10页Plant Physiology Journal

基　　金：国家自然科学基金(31372050);山东省现代农业产业技术体系水果产业创新团队-栽培与设施装备(SDAIT-03-022-05)~~

摘　　要：以‘章姬’草莓为试验材料,研究了喷施5个不同浓度的钼酸钠(Na_2MoO_4,0、1.2、2.4、4.8和9.6 mmol·L^(-1))对氮代谢关键酶活性与^(15)N吸收、分配及利用的影响。结果表明,2.4和4.8 mmol·L^(-1)处理的幼苗的总干重、叶片干重和茎部干重显著高于其他处理,叶片和根部钼浓度随施钼量的增加而显著升高。培养15 d内,2.4 mmol·L^(-1)处理的叶片和根系的硝酸还原酶、亚硝酸还原酶、谷氨酰胺合成酶和谷氨酸合酶活性高于其他处理。各个处理的^(15)N主要分配在叶片中,合适浓度的钼处理(2.4 mmol·L^(-1))的叶片^(15)N分配率最高,高浓度的钼处理(9.6 mmol·L^(-1))的最低。2.4 mmol·L^(-1)处理的全氮量、^(15)N吸收总量、叶片和根系从肥料吸收的^(15)N(Ndff值)以及^(15)N利用率均达到最高。因此,2.4 mmol·L^(-1)处理最有利于草莓幼苗的生长、提高氮代谢关键酶活性和^(15)N吸收、分配和利用。This experiment was carried out to study the effects of sodium molybdate（Na_2MoO_4） foliar sprays on key enzymes activities involved in nitrogen metabolism and ^（15）N absorption, distribution and utilization, using ‘Akihime＇ strawberry seedlings as experiment materials. Na_2MoO_4 levels were 0, 1.2, 2.4, 4.8, 9.6 mmol·L^（-1）. Results showed that total dry weight, dry weight of leaves and stems of 2.4 and 4.8 mmol·L^（-1） treatment were higher than those of the other three treatments. Nitrate reductase, nitrite reductase, glutamine synthase, and NADH-glutamate synthase activities in leaves and roots of 2.4 mmol·L^（-1） treatment showed higher than the other treatments within 15 days. ^（15）N was mainly distributed in leaves of all treatments. The highest ^（15）N distribution was found in 2.4 mmol·L^（-1） treatment and the lowest in 9.6 mmol·L^（-1） treatment. The ^（15）N absorption, the total N, the Ndff values of leaves and roots, and the ^（15）N utilization ratio of 2.4 mmol·L^（-1） treatment were the highest among all the treatments. These results show that 2.4 mmol·L^（-1） treatment is beneficial to strawberry seedlings growth and enhance key enzymes activities about nitrogen metabolism, ^（15）N absorption, distribution and utilization.

关 键 词：钼 草莓 氮代谢 ^(15)N 

分 类 号：S668.4[农业科学—果树学]