两个箭舌豌豆品种根响应镉胁迫的蛋白质组学分析  

Proteomics analysis of cadmium stress responses in the roots of two Vicia sativa varieties differing in Cd tolerance

作　　者：芮海云[1,2] 庄凯[2] 沈振国[2] 张芬琴[3] 

机构地区：[1]泰州学院医药与化学化工学院,江苏泰州225300 [2]南京农业大学生命科学学院,南京210095 [3]河西学院农业与生物技术学院,甘肃张掖734000

出　　处：《植物生理学报》2016年第7期1089-1098,共10页Plant Physiology Journal

基　　金：国家自然科学基金(31160053和31560072);泰州学院博士基金(TZXY2015JBJJ005)~~

摘　　要：为探讨箭舌豌豆根响应镉胁迫的分子机理,以两个镉耐性差异的箭舌豌豆品种(镉耐性品种‘L3’,镉敏感品种‘ZM’)为材料,通过双向电泳和基质辅助激光解析/电离时间飞行质谱技术以及NCBI数据库搜索,分析25μmol·L^(-1) CdCl_2处理5 d后两个品种根蛋白质的差异表达。结果表明,镉胁迫下‘L3’和‘ZM’根共有56个蛋白点的表达量发生显著变化,其中5个蛋白点在两个品种根中共同上调,1个蛋白点共同下调,4个蛋白点为镉处理后两个品种中共同新出现。28个差异表达蛋白点得到鉴定,这些蛋白质的功能可分为七类:(1)抗氧化与解毒相关蛋白;(2)呼吸与能量代谢相关蛋白;(3)胁迫响应相关蛋白;(4)信号转导相关蛋白;(5)细胞壁合成相关蛋白;(6)细胞增殖相关蛋白;(7)未知功能蛋白。镉胁迫下耐性品种‘L3’较敏感品种‘ZM’鉴定到更多抗氧化解毒、胁迫响应和细胞增殖蛋白的上调表达,这可能是耐性品种‘L3’较敏感品种‘ZM’具有更强镉耐性的重要原因;生长素、水杨酸和乙烯诱导的信号转导途径可能参与箭舌豌豆对镉胁迫的响应。In order to understand the molecular mechanisms involved in cadmium stress response, the differentially expressed proteins in the roots of a Cd-tolerant variety ‘L3＇ and a Cd-sensitive variety ‘ZM＇ of Vicia sativa in response to Cd were analyzed by 2-DE, and the functions of these differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS） analysis and NCBI database searching. Seedlings were exposed to 25 μmol·L^（-1） CdCl_2 for 5 days with 0 μmol·L^（-1） CdCl_2 as the control. The results showed that 56 protein spots were differentially expressed in the roots in at least one variety. Five spots were up-regulated and one spot was down-regulated in both varieties. Four spots were newly appeared in both varieties after Cd treatment. Twenty-eight protein spots were identified. The biological functions of these identified proteins were involved in seven metabolic pathways：（1） antioxidative defense and detoxification;（2） respiration and energy metabolism;（3） stress response;（4） cell wall synthesis;（5） signal transduction;（6） cell proliferation; and（7） unknown. Compared with ‘ZM＇, ‘L3＇ had more up-regulated proteins involved in antioxidative defense and detoxification, stress response and cell proliferation, and this may be an important cause of more tolerance of ‘L3＇ than ‘ZM＇. Auxin, salicylic acid and ethylene-induced signal transduction pathway may be involved in V. sativa responses to cadmium stress.

关 键 词：箭舌豌豆 镉 根 蛋白质组学 

分 类 号：S54[农业科学—作物学]