番茄早疫病菌对啶菌噁唑敏感基线建立及药剂作用方式初探  

Sensitivity baseline of Alternaria solani to pyrisoxazole and the mode of action of pyrisoxazole

作　　者：史晓晶[1] 周恩超[1] 任璐[2] 韩巨才[2] 梁彬[1] 刘慧平[2] 

机构地区：[1]忻州师范学院生物系,山西忻州034000 [2]山西农业大学农学院,山西太谷030801

出　　处：《农药学学报》2016年第4期453-458,共6页Chinese Journal of Pesticide Science

基　　金：山西省科技基础条件平台建设项目(2014091003-0102);山西省农业技术推广示范行动项目(SNJTGSFXD201402);忻州师范学院2013年院级青年基金项目(QN201320)

摘　　要：从中国山西、内蒙古、山东、海南、江苏、福建和陕西省（自治区）不同市、县从未使用过啶菌噁唑的露地或保护地采集并经单孢分离获得166株番茄早疫病菌,采用菌丝生长速率法测定了病原菌群体对啶菌噁唑的敏感性。结果表明：啶菌噁唑对番茄早疫病菌的EC50值范围在0.11~2.29 mg/L之间,敏感性频率呈正态分布,其均值（0.56±0.39）mg/L可作为番茄早疫病菌对啶菌噁唑田间抗性监测的敏感基线;不同采集地菌株对啶菌噁唑的敏感性存在差异,其中采自福建省的菌株敏感性最高,而山西省大同、晋中和长治市的菌株敏感性最低。200 mg/L啶菌噁唑对番茄早疫病防治具有优异的保护和治疗作用,且接菌前4 d内施药和接菌后1~2 d内施药防效最佳。研究表明,啶菌噁唑对番茄早疫病防治具有良好的实际应用前景。To study the sensitivity of Alternaria solani to pyrisoxazole, 166 single-spore isolates were collected from tomato fruits or leaves infected by A. solani without exposure to pyrisoxazole in open field or greenhouse in Shanxi province, Inner Mongolia Autonomous Region, Shandong province,Hainan province, Jiangsu province, Fujian province and Shaanxi province. The sensitivity of each isolate to pyrisoxazole was determined by mycelial growth inhibition rate. The range of EC50 values were from 0.11 mg/L to 2.29 mg/L, and the sensitivity frequency was normally distributed. Thus, the sensitivity baseline was established with the mean EC50 value（0.56 ± 0.39） mg/L of pyrisoxazole. There were significant differences in the mean EC50 value of each collected district. The isolates from Fujian province were the most sensitive, while the isolates from Shanxi province（Datong, Jinzhong and Changzhi） were the least sensitive to pyrisoxazole. Pyrisoxazole provided excellent protective and curative activity on A. solani when applied at 200 mg/L, and it showed the best control efficacy before the artificial inoculation in 4 days and after inoculation in 2 days. Consequently, there is a good application prospect to pyrisoxazole for controlling tomato early blight.

关 键 词：番茄早疫病菌 啶菌噁唑 敏感基线 作用方式 保护作用 治疗作用 

分 类 号：S482.2[农业科学—农药学] S481.4[农业科学—植物保护]