消银汤对银屑病小鼠模型血清中IFN-γ和IL-4的影响  

Effect of Xiaoyin Decoction on serum IFN-γand IL-4 in psoriasis mouse model

作　　者：宋红霞[1] 薛晓东[2] 

机构地区：[1]重庆三峡医药高等专科学校药理教研室,404000 [2]重庆三峡医药高等专科学校附属医院皮肤科,404000

出　　处：《检验医学与临床》2016年第15期2109-2110,2113,共3页Laboratory Medicine and Clinic

基　　金：重庆市万州区科技计划项目(No.201303042)

摘　　要：目的观察消银汤治疗银屑病小鼠模型的作用,并探讨其可能的作用机制。方法建立小鼠阴道上皮模型,设消银汤高、中、低剂量组,甲氨蝶呤组和生理盐水组,观察各组对小鼠阴道上皮细胞有丝分裂的影响,并采用酶联免疫吸附测定(ELISA)法检测各组小鼠血清中γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)的水平。结果与对照组比较,消银汤高、中、低剂量组均可明显抑制小鼠阴道上皮的有丝分裂,并均可降低IFN-γ水平,且差异具有统计学意义(P<0.05),高剂量组可明显升高IL-4水平,差异具有统计学意义(P<0.01)。结论消银汤具有抑制表皮细胞增殖的作用,可能通过降低血清中IFN-γ水平,升高IL-4水平来发挥治疗作用。Objective To observe the effect of Xiaoyin Decoction in treating psoriasis mouse model and to investigate its possible mechanism .Methods The mouse vaginal epithelial model was established .The model mice were divided into the high ,middle and low doses of Xiaoyin Decoction groups ,methotrexate（MTX） group and normal saline group .The effects of Xiaoyin Decoction on the vaginal epithelial cell mitosis were evaluated and the levels of serum IFN‐γ and IL‐4 were detected by ELISA .Results Compared with the control group ,high ,middle and low doses of Xiaoyin Decoction groups could remarkable inhibit the mouse vaginal epithelial cell mitosis and decreased the IFN‐γlevel ,the differences were statistically significant （P〈0 .05） ,the high dose group could signifi‐cantly increase the IL‐4 level ,the difference was statistically significant （P〈0 .01） .Conclusion Xiaoyin Decoction has the effect for inhibiting epidermal cell proliferation ,which plays the curative effect possibly by decreasing the serum IFN‐γlevel and increas‐ing the serum IL‐4 level .

关 键 词：银屑病 消银汤 干扰素-Γ 白细胞介素-4 

分 类 号：R285.5[医药卫生—中药学]