益气活血复方对慢性心衰大鼠心肌能量代谢mi-CK mRNA及蛋白的影响  

Effect of Yiqi Huoxue Formula on Expression of mi-CK mRNA and Protein in Rats with Chronic Heart Failure

作　　者：廖佳丹[1] 王鹏程[2] 张艳[3] 杨炜[1] 吴作跃[1] 姜钧文[3] 

机构地区：[1]浙江中医药大学附属第三医院,浙江杭州310000 [2]杭州市红十字会医院,浙江杭州310003 [3]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2016年第8期1968-1971,I0011,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省科技厅项目(2012020108-211)

摘　　要：目的:通过观察益气活血复方对心衰大鼠心肌组织mi-CK mRNA及蛋白表达的影响,从能量代谢角度研究中药在慢性心衰治疗中的作用。方法:本实验以冠脉结扎法配合力竭式游泳、减食等方法造成大鼠慢性心衰动物模型,对造模成功大鼠分为模型组、西药组、中药组,没有进行左冠脉结扎手术的假手术大鼠为正常组。检测各组大鼠±dp/dt max、心肌组织mi-CK mRNA及蛋白表达水平。结果:与模型组比较,各药物组±dp/dt max、mi-CK mRNA及蛋白表达均升高,差异有统计学意义(P<0.05)。与西药组比较,中药组±dp/dt max升高明显,差异有统计学意义(P<0.05)。在mi-CK mRNA及蛋白表达方面,两治疗组间比较,差异无统计学意义(P>0.05)。结论:益气活血复方可以增加心衰大鼠心肌mi-CK mRNA及蛋白的表达、改善ATP转运穿梭效率,起到修复心衰心肌能量代谢的作用。Objective： To observe the effect of Yiqi Huoxue Formula on the expression of mitochondrial creatinekinase（ mi-CK） mRNA and protein in rats with chronic heart failure and study the role of traditional Chinese medicine in the treatment of chronic heart failure from the perspective of energy metabolism. Methods： The experiment used coronary artery ligation with underfeed and exhausted swimming to set up the chronic heart failure rats model. Divide CHF rats into model group,western medicine control group,traditional Chinese medicine group and no left coronary artery ligation normal group. Observe the expression of mi-CK mRNA and protein around infarction area. Results： The expressions of ± dp / dt max,mi-CK mRNA and protein in medication group were increased obviously. The difference in model group and medicine control groups was statistically significant（ P 〈 0. 05）. Compared with western medicine control group,the effect on raising ± dp / dt max of traditional Chinese medicine group was much better than that of the western medicine control group and the difference was significant（ P 〈 0. 05）. Two medicine groups’ expressions of mi-CK mRNA and protein were not statistically significant（ P 〉 0. 05）. Conclusion： Yiqi Huoxue Formula can effectively increase the expression level of mi-CK mRNA and protein in chronic heart failure rats,improving ATP transfer efficiency,and thus repairing the myocardial energy metabolism.

关 键 词：益气活血复方 能量代谢 线粒体肌酸激酶 慢性心衰 

分 类 号：R285.5[医药卫生—中药学]