消退期瘢痕与成熟期瘢痕组织中lncRNA表达谱的差异分析  

Differential expression profile of long non-coding RNA in regressive scar and mature scar

作　　者：李俊[1] 李倩[1] 高艳丽[1] 周蓓[1] 李景云[1] 

机构地区：[1]南京医科大学附属南京妇幼保健院医疗美容科,210004

出　　处：《中华整形外科杂志》2016年第4期298-302,共5页Chinese Journal of Plastic Surgery

基　　金：江苏省自然科学基金（BK20140083）

摘　　要：目的 分析长链非编码RNA （lncRNA）在消退期瘢痕及成熟期瘢痕组织中的表达差异.方法 通过HE染色、Masson染色观察消退期瘢痕及成熟期瘢痕的组织形态.利用lncRNA芯片技术分别检测消退期瘢痕及成熟期瘢痕组织中差异表达的lncRNA,通过对原始数据进行预处理达到均一化后,筛选出差异较大的lncRNA并进行靶基因预测及信号通路分析.实时荧光定量PCR验证其中可能与瘢痕相关的lncRNA.结果 与成熟期瘢痕组织相比较,消退期瘢痕组织中成纤维细胞的含量较多,Masson染色颜色较深,lncRNA升高3倍以上的共l 275条,降低70％以上的共596条.信号通路分析提示细胞因子信号通路、TNF信号通路、胰岛素分泌以及细胞黏连通路在瘢痕发生、发展中发挥着重要作用.实时荧光定量PCR验证的结果与IncRNA芯片分析结果的趋势基本一致.结论 与成熟期瘢痕组织相比较,消退期瘢痕组织中lncRNA表达谱发生明显变化,提示IncRNA可能在瘢痕成熟的过程中发挥重要作用.Objective To analyze the expression profile of long non-coding RNA （lncRNA） in regressive scar and mature scar.Methods Hematoxylin-eosin staining and Masson staining were used to examine the histology of regressive scar and mature scar.The technique of IncRNA microarray was used to test the IncRNA expression profile in regressive scar and mature scar.The raw data of lncRNA were pretreated for normalization.Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis was further performed.Four lncRNA expressions were validated by quantitative PCR.Results Compared with mature scar,more fibroblasts and deeper Masson staining color were seen in the regressive scar,a total of 1 275 upregulated more than 3-fold were found in the regressive scar.And 596 downregulated more than seventy percent lncRNA were found in the regressive scar.Pathway analysis indicated that chemokine signaling pathway,TNF signaling pathway,insulin secretion and focal adhesion would play important roles in scar development.Conclusions lncRNA expression profiles in the regressive scar is markedly different in comparison with the mature scar,revealing that lncRNA may participate in pathophysiological process of scar maturation.

关 键 词：寡核苷酸序列分析 瘢痕 

分 类 号：R622[医药卫生—整形外科]