顺丁烯二酸酐-β-环糊精毛细管电色谱整体柱的制备及应用  

Preparation and Application of Maleic Anhydride-β-cyclodextrin Monolithic Column for Capillary Electrochromatography

作　　者：李英杰[1] 刘树仁[1] 高立娣[1] 吕仁江[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《化学通报》2016年第8期749-753,共5页Chemistry

基　　金：黑龙江省自然科学基金项目(B201218);齐齐哈尔大学研究生创新科研项目(YJSCX2015-017X)资助

摘　　要：以顺丁烯二酸酐-β-环糊精(MAH-β-CD)、甲基丙烯酸缩水甘油酯(GMA)和二甲基丙烯酸乙二醇酯(EDMA)为功能单体,一步键合制备了环糊精衍生物毛细管整体柱,并对其制备条件进行优化。通过红外光谱和扫描电镜对整体柱固定相的结构和形貌进行表征。以硫脲为中性标记物测得柱效为99856 plates/m。通过精氨酸对柱子进行评价,所制备的新型手性整体柱具有较强的手性拆分能力,且重现性好、操作简单。用毛细管电色谱-质谱联用技术(CEC-MS)拆分了沙丁胺醇对映体,分离度为1.67。Maleic anhydride-β-cyclodextrin chiral monolithic capillary electrochromatography column was prepared by one-step reaction with maleic anhydride-β-cyclodextrin（ MAH-β-CD）,glycidyl methacrylate（ GMA） and ethylene glycol dimethacrylate（ EDMA） as functional monomers. Moreover, the preparation conditions of the monolithic column were also optimized. The structure and morphology of the monolithic column were characterized by IR and SEM. The column efficiency determined by using thiourea as the neutral marker was 99856 plates / m. The column was evaluated by arginine. The new monolithic column had good chiral recognition ability, better reproducibility and easy operation. In capillary electrochromatography mode, the Salbutamol enantiomers were separated by capillary electrochromatography-mass spectrometry（ CEC-MS）,and the resolution is 1. 67.

关 键 词：整体柱 顺丁烯二酸酐-β-环糊精 毛细管电色谱-质谱 对映体拆分 

分 类 号：O652.63[理学—分析化学]