PCR-Array技术研究梅毒患者血清固定Th17免疫调节网络相关基因的表达  

Expression profiles of Th17 pathway-related genes in patients with syphilis serofast reaction analyzed by PCR-Array technique

作　　者：关杨[1] 陶小华 蓝丽娜[1] 吴肖冰[1] 杨帆[1] 洪福昌[1] 

机构地区：[1]深圳市慢性病防治中心,518020 [2]江西省皮肤病专科医院,南昌330000

出　　处：《中华微生物学和免疫学杂志》2016年第7期523-527,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81360260）;广东省医学科研基金（A2014650）

摘　　要：目的：研究梅毒患者血清固定Th17细胞免疫调节网络相关基因的表达,探讨Th17细胞在梅毒患者血清固定中的作用。方法收集梅毒患者血清固定、治疗后血清阴转及正常人外周血各8例,提取总RNA,逆转录合成cDNA,采用实时定量聚合酶链反应Array法（ PCR-Array）法检测Th17细胞免疫调节网络相关84种基因mRNA表达水平。结果梅毒血清固定、血清转阴患者和正常对照组3组间PCR-Array所测的Th17免疫调节网络相关基因表达水平两两对比,以差异表达（上调或下调）大于2倍的基因作为有意义的差异表达基因：（1）梅毒血清固定组与正常对照组比较,2种基因（Foxp3、IL-10）表达上调,13种基因（CCL22、CSF2、CSF3、CXCL6、IL-17A、IL-17D、IL-21、IL-23R、IL-9、IRF4、RORA、RORC、STAT3）表达下调；（2）梅毒血清固定组与血清阴转组比较,2种基因（Foxp3、IL-10）表达上调,9种基因（CCL22、CSF2、CSF3、IL-17A、IL-21、IL-23R、IRF4、RORC、STAT3）表达下调；（3）血清阴转组与正常对照组比较,2种基因（CXCL6、IL-9）表达下调。结论 Th17细胞免疫调节网络中异常表达的基因可能与梅毒血清固定相关。Objective To detect the expression of Th17 pathway-related genes in patients with syphilis serofast reaction and to investigate the mechanism of Th17 cells in syphilis serofast reaction. Meth-ods Peripheral blood samples were collected from patients with syphilis serofast reaction （ n=8 ） , patients who were syphilis-seronegative after treatment （n=8） and healthy subjects （n=8）. Total RNA was extrac-ted from each blood sample and then reversely transcribed into cDNA. PCR-Array analysis was performed to quantify the expression levels of Th17 pathway-related genes. Results The expression levels of genes with a fold change 〉2 （up or down regulated） were defined as differentially expressed. （1） Compared with the control group, the patients with syphilis serofast reaction showed increased expression of genes encoding fork-head box protein 3 （Foxp3） and IL-10, but decreased expression of genes encoding C-C motif chemokine 22 （CCL22）, colony stimulating factor 2 （CSF2）, CSF3, chemokine （C-X-C motif） ligand 6 （CXCL6）, IL-17A, IL-17D, IL-21, IL-23R, IL-9, interferon regulatory factor 4 （IRF4）, RAR-related orphan receptorα（ RORα） , RAR-related orphan receptor γ （ RORγ） and signal transducer and activator of transcription 3 （STAT3）. （2） Compared with the seronegative syphilis group, the expression levels of genes encoding Foxp3 and IL-10 in patients with syphilis serofast reaction were up-regulated, while the expression of genes encoding CCL22, CSF2, CSF3, IL-17A, IL-21, IL-23R, IRF4, RORγ and STAT3 were down-regulated. （3） The expression levels of genes encoding CXCL6 and IL-9 in seronegative syphilis group were lower than those in control group. Conclusion The abnormal expression of Th17 pathway-related genes might relate to the pathogenesis of serofast state of syphilis.

关 键 词：梅毒 血清固定 TH17细胞 寡核苷酸序列分析 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]