活性维生素D_3对大鼠肾小球系膜细胞增殖影响  

Effect of 1,25-dihydroxyvitamin D_3 on proliferation of rat glomerular mesangial cells

作　　者：张春江[1] 陈建平[2] 赵丹[1] 陈志刚[3] 杨晓萍[1] 

机构地区：[1]石河子大学医学院第一附属医院肾病科,新疆石河子832008 [2]石河子大学医学院 [3]石河子大学医学院第一附属医院中心实验室

出　　处：《中国公共卫生》2016年第8期1062-1065,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81160090);新疆石河子大学博士基金(RCZX201026)

摘　　要：目的探讨活性维生素D3(1,25(OH)2D3)对大鼠系膜细胞增殖的影响。方法体外培养大鼠系膜细胞,随机分为正常对照组、表皮生长因子(EGF)(10 ng/m L)组、1,25(OH)2D3(10-8mol/L)组、EGF联合1,25(OH)2D3组,采用MTT法检测各组干预48 h后对大鼠系膜细胞增殖的影响,流式细胞术检测各组干预48 h后大鼠系膜细胞周期分布情况,免疫荧光法检测各组干预48 h后,大鼠系膜细胞中增生性细胞核抗原(PCNA)的表达情况。结果与正常对照组比较,EGF组能明显促进大鼠系膜细胞增殖,G0/G1期细胞减少,S期及G2/M期细胞增加,且PCNA表达增加,1,25(OH)2D3组大鼠系膜细胞增殖受抑制,G0/G1期细胞增加,S期及G2/M期细胞减少,且PCNA表达降低;与EGF组比较,EGF联合1,25(OH)2D3干预组系膜细胞增殖受抑制,G0/G1期细胞增多,S期及G2/M期细胞减少,且PCNA表达降低。结论 1,25(OH)2D3可阻滞大鼠系膜细胞的细胞周期并抑制大鼠系膜细胞的增殖和EGF对大鼠系膜细胞的促增殖作用。Objective To explore the effect of 1,25-dihydroxyvitamin D3（ 1,25[OH]2D3） on the proliferation of cultured rat glomerular mesangial cells. Methods The cultured rat glomerular mesangial cells were divided into four groups： normal control,epidermal growth factor（ EGF）（ 10 ng / m L）,1,25（ OH）2D3（ 10- 8mol / L）,and EGF plus 1,25（ OH）2D3group and all the cells were treated for 48 hours. The cell proliferation was measured with methylthiazolyldiphenyl-tetrazolium bromide（ MTT） colorimetric method; the cell cycles were measured with flowcytometry; and the expression of proliferating cell nuclear antigen（ PCNA） was measured with immunofluorescence staining. Results Compared with those of the normal control group,the proliferation of rat glomerular mesangial cells in EGF group was promoted,with a decrease in the ratio of the cells in G0/ G1 phase and an increase of cells in S and G2/ Mphases,and the expression of PCNA was increased. The proliferation of rat glomerular mesangial cells in 1,25（ OH）2D3group was inhibited,with an increased ratio of cells in G0/ G1 phase and a decreased ratio of cells in S and G2/ Mphases,and the expression of PCNA was decreased. Compared to those of the EGF-only treatment group,the proliferation of rat glomerular mesangial cells in EGF plus 1,25（ OH）2D3group was inhibited,with an increased ratio of cells in G0/ G1 phase and a decreased ratio of cells in S and G2/ Mphases,and the expression of PCNA was decreased. Conclusion Treatment with 1,25（ OH）2D3can significantly impact cell circle and inhibit both the proliferation of rat glomerular mesangial cells and the promotional effect of EGF on the proliferation of rat glomerular mesangial cells.

关 键 词：1 25(OH)2D3 肾小球系膜细胞 表皮生长因子(EGF) 细胞增殖 增生性细胞核抗原(PCNA) 

分 类 号：R692[医药卫生—泌尿科学]