聚氨酯/胶原蛋白复合纳米纤维支架的性能  

Performance of composite polyurethane/collagen nanofiber scaffolds

作　　者：贾琳[1] 陈莉娜[1] 张海霞[1] 覃小红[1,2] 

机构地区：[1]河南工程学院纺织学院,河南郑州450007 [2]东华大学纺织学院,上海201620

出　　处：《纺织学报》2016年第8期1-6,共6页Journal of Textile Research

基　　金：河南省重点科技攻关项目(152102210301);河南省高校科技创新团队支持计划项目(13IRTSTHN024;15IRTSTHN011);河南省高校重点科研项目(15A540001);河南工程学院博士基金项目(D2014025)

摘　　要：针对聚氨酯(PU)力学性能较好,亲水性较差,细胞在其表面的黏附较少,不适宜单独作为血管组织工程支架材料的问题,为提高PU纳米纤维支架的亲水性和生物相容性,通过静电纺丝制备不同比例的复合聚氨酯/胶原蛋白纳米纤维支架,利用扫描电镜、红外光谱仪等观察测试纳米纤维支架材料的形态结构、化学性能等;并在纳米纤维支架表面培养血管平滑肌细胞,通过扫描电镜观察细胞在支架表面的生长情况。结果表明:胶原蛋白的加入使纤维直径从453 nm减小到了154 nm,平均孔径尺寸从0.64μm降低到了0.28μm,而且增加了细胞在其表面的黏附和增殖;当聚氨酯和胶原蛋白的质量比为3∶1时,纳米纤维支架的拉伸强度最大,生物相容性最好,表面黏附生长的细胞最多。Polyurethane has excellent mechanical property,however,pure PU is not suitable as vascular tissue engineering scaffold due to its hydrophobicity and less cells were attached on its surface.In this paper,composite polyurethane/collagen nanofibrous scaffolds were prepared by electrospinning to enhance the hydrophilicity and biocompatibility of PU nanofibrous scaffolds.Firstly,the morphologies and chemical and mechanical characterization were carried out using scanning electron microscopy（SEM）and Fourier transform infrared,respectively.Then smooth muscle cells（SMCs） were cultured on nanofibrous scaffolds and the attachment and proliferation of cells were evaluated using（SMCs））.The results show that the addition of collagen decreased the diameter of nanofibers from 453 nm to 154 nm,decreased the pore diameter from 0.64 μm to 0.28 μm,and enhance the proliferation and attachment of cells.When the mass ratio of polyurethane and collagen is 3∶ 1,the tensile strength and biocompatibility of composite PU/collagen（3∶ 1） scaffolds were the best,hence,more SMCs were observed on this scaffold.

关 键 词：复合纳米纤维 静电纺丝 血管组织工程 聚氨酯 

分 类 号：TS102.6[轻工技术与工程—纺织工程]