Th17／调节性T淋巴细胞比率在急性乙型肝炎患者外周血的动态变化及其意义  

Dynamic change in Th17/regulatory T cell ratio in peripheral blood of patients with acute hepatitis B and its significance

作　　者：程雪[1] 单永业 罗亚文[3] 

机构地区：[1]济宁市传染病医院,272000 [2]甘肃省第二人民医院,730000 [3]遵义医学院附属医院感染科,563003

出　　处：《中华肝脏病杂志》2016年第8期565-568,共4页Chinese Journal of Hepatology

基　　金：贵州省科技厅社会发展攻关项目（黔科合SY字[2011]3048）

摘　　要：目的 观察急性乙型肝炎（AHB）患者外周血Th17（CD3’CD8IL-17＋）、调节性T淋巴细胞（Treg，CD4＋CD25＋CD127low）细胞频率及Th17／Treg比率的动态变化，探讨其与AHB疾病转归的关系。方法选择10例AHB患者为观察组，据疾病转归分为急性期、恢复早期、痊愈后，10名健康体检者为对照组。采用多色流式细胞术检测各期外周血中Th17、Treg细胞频率，计算Th17／Treg比率；全自动生物化学分析仪检测肝功能，RT-PCR检测HBVDNA水平。组间比较采用单因素方差分析，进一步两两比较采用f检验。结果AHB急性期Th17、Treg细胞频率（18．22％±4．13％、6．46％±2．46％）及Th17／Treg比率（3．37土1．73）均较健康对照组（0．68％±0．29％、1．62％±0．18％、0．42％±0．20％）升高护值均〈0．01）。恢复早期与急性期相比，Th17细胞频率（3．14％±1．90％）下降沪〈0．01）、Treg细胞频率（11．73％±1．76％）上升垆〈0．01），Th17／Treg比率（0．27±0．19）下降垆〈0．01）；痊愈后与恢复早期相比，Th17细胞频率（3．31％±0．95％）稍有上升妒=0．888）；Treg细胞频率（6．83％±1．85％）下降（P〈0．01），但仍高于对照组P〈0．01）；Th17／Treg比率（0．52±0．21）稍有上升垆〈0．05），但与健康对照比较未见明显差异（P=0．286）。在AHB急性期，HBsAg、HBeAg定量水平与Th17／Treg比率均存在正相关关系（r=0．639，P〈0．05；r=0．633，P〈0．05）。结论Th17、Treg细胞频率和Th17／Treg比率的动态变化可能与AHB患者疾病转归有关。Objective To investigate the dynamic changes in the frequencies ofTh17 （CD3＋CD8- IL-17） and regulatory T cells （Treg, CD4＋CD25＋CD127low） and Thl7/Treg ratio in the peripheral blood in patients with acute hepatitis B （AHB）, as well as their association with the outcome of AHB. Methods A total of 10 AHB patients were enrolled as observation group, and according to the outcome of AHB, these patients were further divided into acute stage group, early recovery group, and full recovery group. Another 10 healthy subjects who underwent physical examination were enrolled as control group. Flow cytometry was used to measure the frequencies of Th17 and Treg in peripheral blood, and the Th17/Treg ratio was calculated. An automatic biochemical analyzer was used to measure liver function parameters, and RT-PCR was used to measure HBV DNA. An one-way analysis of variance was used for comparison between groups, and the t-test was used for comparison between any two groups. Results Compared with the control group, the acute stage group showed significant increases in the frequencies of Thl7 and Treg （18.22%±4.13%/6.46%±2.46% vs 0.68%±0.29%/1.62% ±0.18%, P 〈 0.01） and the Thl7/Treg ratio （3.37±1.73 vs 0.42＋0.20, P 〈 0.01）. Compared with the acute stage group, the early recovery group showed a significant reduction in the frequency of Thl 7 （3.14%±1.90%, P 〈 0.01）, a significant increase in the frequency of Treg （11.73%±1.76%, P 〈 0.01）, and a significant reduction in the Th 17/Treg ratio （0.27±0.19, P 〈 0.01）. Compared with the early recovery group, the full recovery group showed a slight increase in the frequency of Th17 （3.31±0.95, P = 0.888）, a significant reduction in the frequency of Treg （6.83%±1.85%, P 〈 0.01）, but which was significantly higher than that in the control group （P 〈 0.01）, and a slight increase in the Thl7/Treg ratio （0.52±0.21, P 〈 0.05）, which showed no significant difference between this group and the control group （P = 0.

关 键 词：肝炎 乙型 急性 T淋巴细胞 调节性 TH17 

分 类 号：R512.62[医药卫生—内科学]