基于同位素标记相对和绝对定量技术筛选乙型肝炎慢加急性肝衰竭患者的血清生物标志物  

Screening of serum biomarkers by isobaric tags for relative and absolute quantitation in patients with HBV- related acute - on - chronic liver failure

作　　者：刘慧敏[1] 高方媛[1] 于浩[1] 孟培培[1] 江宇泳[1] 王宪波[1] 

机构地区：[1]首都医科大学附属北京地坛医院中西医结合中心,100015

出　　处：《中华肝脏病杂志》2016年第8期580-584,共5页Chinese Journal of Hepatology

基　　金：北京市中医药科技项目（QN2013-14）;国家自然科学基金（81503428）;首都医学科学发展基金（2016-4-2172）;北京市卫生优秀人才（2015000021469G203）;北京地坛医院院内课题（DTQL201405）

摘　　要：目的 采用核素标记相对和绝对定量（iTRAQ）技术筛选乙型肝炎慢加急性肝衰竭（HBV-ACLF）患者的血清生物标志物。方法本实验采用凝胶电泳法分离、去除高丰度蛋白质，用iTRAQ试剂标记每组肽段后采用UltiMateTM3000纳升高效液相色谱仪、Q—Exactive串联质谱仪进行检测，ProteinDiscovery软件分析质谱数据，并对差异表达的蛋白质进行生物信息学分析。结果HBV-ACLF组、慢性乙型肝炎（CHB）组各纳入10例样本，HBV—ACLF组生存组和死亡组各纳入6例样本。与CHB组比较，HBV—ACLF组差异表达的蛋白共有43个，其中下调的34个，上调的9个；与HBV—ACLF生存组比较，死亡组的差异蛋白共33个，其中上调18个，下调15个。结论血清中鉴定获得的角蛋白、α1-酸性糖蛋白、锌-α2-糖蛋白可能是判断HBV—ACLF预后潜在的生物标志物，值得进一步深入研究。Objective To investigate the screening of serum biomarkers in patients with HBV-related acute-on-chronic liver failure （HBV-ACLF） by isobaric tags for relative and absolute quantitation （iTRAQ）. Methods Gel electrophoresis was used to isolate and remove high-abundant proteins. Each group of peptides was labeled by the iTRAQ reagents and then tested with an UltiMateTM 3000 nanoliter high-performance liquid chromatograph, and a Q-Exactive tandem mass spectrometer. The Protein Discovery software was used to analyze mass spectrometry data and perform bioinformatie analysis for differentially expressed proteins. Results Ten samples each were included in the HBV-ACLF group and the chronic hepatitis B （CHB） group, and six samples each were included in the HBV-ACLF survival group and the HBV-ACLF death group. Compared with the CHB group, the HBV-ACLF group had 43 differentially expressed proteins, among which 34 were downregulated and 9 were upregulated. Compared with the HBV-ACLF survival group, the HBV-ACLF death group had 33 differentially expressed proteins, among which 18 were upregulated and 15 were downregulated.Conclusion Keratin, a1-acid glycoprotein, and zinc-a2-glycoprotein identified in the serum may be used as potential biomarkers for predicting the prognosis of patients with HBV-ACLF.

关 键 词：肝功能衰竭 肝炎 乙型 慢性 生物标志物 同位素标记 

分 类 号：R512.62[医药卫生—内科学] R575.3[医药卫生—临床医学]