自噬在大鼠炎性痛形成中的作用  

Role of autophagy in development of inflammatory pain in rats

作　　者：张桂友[1] 王准[2] 杨艳梅[2] 栾静[2] 郑莹[2] 魏成博 吕丹[2] 

机构地区：[1]遵义医学院附属医院麻醉科,563000 [2]天津市第一中心医院疼痛科,300192

出　　处：《中华麻醉学杂志》2016年第4期471-473,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81200851）;天津市科委基金项目（05YFJMJC14800）;天津市卫生局攻关项目（13KG105）

摘　　要：目的 评价自噬在大鼠炎性痛形成中的作用。方法 健康成年雄性SD大鼠24只，体重180～240 g，采用随机数字表法分为4组（n＝6）：对照组（C组）、炎性痛组（IP组）、二甲基亚砜组（D组）和自噬诱导剂雷帕霉素组（R组）。采用左侧后肢足底皮下注射蜜蜂毒溶液50 μl的方法制备大鼠慢性炎性痛模型。C组左侧后肢足底皮下注射0.9%无菌生理盐水；D组给予2%亚甲基亚砜灌胃3 d，1 ml/d，于第3天灌胃后1 h时制备炎性痛模型；R组给予雷帕霉素10 mg/kg（溶于2%二甲基亚砜中）灌胃3 d，1 ml/d，于第3天灌胃后1 h时制备炎性痛模型。于造模后2 h时测定机械缩足反应阈（MWT）和热缩足潜伏期（TWL）。痛阈测定结束后处死大鼠，取脊髓背角组织，采用Western blot法检测微管相关蛋白轻链3Ⅱ型（LC3Ⅱ）、Beclin-1和p62的表达水平。结果 与C组比较，IP组和D组MWT降低，TWL缩短，IP组、D组和R组脊髓背角组织LC3Ⅱ、Beclin-1和p62的表达上调（P〈0.05或0.01）；与IP组比较，R组MWT升高，TWL延长，脊髓背角LC3Ⅱ和Beclin-1的表达上调，p62表达下调（P〈0.05或0.01），D组上述各指标差异无统计学意义（P〉0.05）。结论自噬障碍参与了大鼠炎性痛的形成。Objective To evaluate the role of autophagy in the development of inflammatory pain in the rats. Methods Twenty-four healthy adult male Sprague-Dawley rats, weighing 180-240 g, were randomly divided into 4 groups （n=6 each） by using a random number table： control group （group C） , inflammatory pain group （group IP） , dimethyl sulfoxide （DMSO） group （group D） , and rapamycin （auto- phagy inducer） group （group R）. Inflammatory pain was produced by injecting 50 p,1 bee venom into the plantar surface of the left hindpaw. In group C, 0.9% normal saline was injected into the plantar surface of the left hindpaw. In group D, 2% DMSO was injected through a gastric tube into the stomach 1 ml per day for 3 consecutive days, and the model was established at 1 h after injection on 3rd day. In group R, rapa- myein 10 mg/kg （in 2% DMSO） was injected through a gastric tube into stomach 1 ml per day for 3 consec- utive days, and the model was established at 1 h after injection on 3rd day. The mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured at 2 h after the model was established. After measurement of the pain threshold, the dorsal horn of the spinal cord was removed for determination of the expression of microtubule-associated protein 1 light chain 3 Ⅱ （LC3 Ⅱ ） , Beclin-1 and p62 by Western blot. Results Compared with group C, the MWT was significantly decreased, the TWL was significantly shortened in IP and D groups, and the expression of LC3 Ⅱ , Beclin-1 and p62 in the dorsal horn of the spinal cord was significantly up-regulated in IP, D and R groups （P〈0.05 or 0.01）. Compared with group IP, the MWT was significantly increased, the TWL was significantly prolonged, the expression of LC3Ⅱ and Beclin-I in the dorsal horn of the spinal cord was significantly up-regulated, and the expression of p62 was significantly down-regulated in group R （P〈0.05）, and no significant change was found in the parameters mentioned above

关 键 词：自噬 炎症 疼痛 

分 类 号：R614[医药卫生—麻醉学]