利用花粉管通道技术将外源银白杨DNA导入黑杨  

Introduction of Exogenous DNA of Populus alba into Sect. Aigeiros via Pollen-tube Pathway

作　　者：赵鑫闻 

机构地区：[1]辽宁省杨树研究所,盖州115200

出　　处：《植物学报》2016年第4期533-541,共9页Chinese Bulletin of Botany

基　　金：辽宁省科学事业公益研究基金(No.2011005007)

摘　　要：黑杨派杨树具有速生丰产、实用性强和无性繁殖能力强等优点;白杨派杨树则具有材质好和抗逆性强等优点。两个派间的许多经济性状优势互补,因此黑杨派与白杨派杂交能够获得多优势性状聚合于一体的优良杂种无性系。但黑杨派与白杨派间杂交天然不育,为了克服黑杨派与白杨派间的远缘杂交障碍,利用花粉管通道技术将银白杨(Populus alba)总DNA导入辽宁杨(Populus×Liaoninggensis)×N001(P.deltoids cv.‘N001’)。获得的D1代植株通过形态学鉴定,发现其中4株明显具有供体银白杨的表型特征。进一步对其进行AFLP分子鉴定,最终确定此4株变异植株均为外源银白杨DNA的阳性转化植株。该研究初步建立了黑杨花粉管通道遗传转化体系,确定外源DNA导入黑杨的最适时间为授粉后30–72小时,适宜方法为不切柱头滴加导入法,最佳浓度为200μg·m L–1。Sect. Aigeiros is fast-growing and practical and has strong agamogenesis ability. Sect. Leuce has good quality and strong resistance. Many economic traits between them are complementary, so Sect. Aigeiros and Leuce hybrid advantages in polymerization can be obtained from a superior hybrid. However, there are natural incompatibility barriers between Sect. Aigeiros and Leuce. In this study, we introduced the genotype of P. alba into P. × Liaoninggensis sp. nov × P. deltoids cv. ‘N001＇ by the pollen-tube pathway to overcome this difficulty. Four of the offspring were similar to P. alba by analysis of phenotype characteristics. From the molecular identification of AFLP, the 4 variant plants were determined to be positive transgenic plants from the genotype of P. alba. Meanwhile, the transformation system of the pollen-tube pathway for Sect. Aigeiros was optimized： the optimal treatment time was 30–72 h, optimal treatment method the uncut stigma method, and optimal treatment 200 μg·m L–1.

关 键 词：FISH-AFLP 银白杨 黑杨组 花粉管通道法 

分 类 号：S792.11[农业科学—林木遗传育种]