鲍曼不动杆菌临床分离株外排泵分布水平与碳氢酶烯类抗生素耐药研究  

Study on the distribution of efflux pump and the antibiotic resistance of carbapenems in clinical isolates of Acinetobacter Bauman

作　　者：李卓成[1] 张彦鹏[1] 吴伟清[1] 李延武[1] 丁芳林[1] 

机构地区：[1]广东省深圳市第二人民医院检验科,518000

出　　处：《重庆医学》2016年第22期3057-3059,共3页Chongqing medicine

基　　金：深圳市科技创新委员会"知识创新计划"项目(JCYJ20140414170821183)

摘　　要：目的研究鲍曼不动杆菌临床分离株中外排泵分布水平与碳氢酶烯类抗生素耐药相关情况。方法选择2015～2016年度深圳地区临床分离的182株鲍曼不动杆菌，同时进行外排泵抑制试验和肉汤稀释法药敏试验，并对结果按照药敏和外排泵表型两种因素分组进行统计学分析。结果鲍曼不动杆菌182株临床分离株中，外排泵表型阳性54株（29．7％）；结合碳氢酶烯类抗生素药敏分组统计，阳性组中耐药和敏感分别为31．9％和13．9%，差异有统计学意义（x2=4．46，P〈0．05）。结论鲍曼不动杆菌外排泵耐药机制在临床分离株的碳氢酶烯类抗生素耐药中起作用。Objective To study the distribution of efflux pump and the antibiotic resistance of carbapenems in clinical isolates of 182 strains of Bauman Acinetobacter,and to understand the correlation between distribution of efflux pump and the drug resistance of hydrocarbon. Methods Totally 182 clinical isolates of Acinetobacter Baumannii were selected to detect the efffux phenotype, which were separated between 2015 to 2016 isolated from Shenzhen district, Guangdong province. Efflux pump inhibition test and broth dilution method drug sensitivity test were used. According to the results of the drug sensitivity and the phenotype of efflux pump,Acinetobacter Baumannii were divided into groups （carbapenems resistance group, carbapenems sensitive group, el＇flux pump positive group and efflux pump negative group） ,then the results were made statistically analysis. Results In all the 182 isolates,there were 54 strains with positive efflux pump phenotype,and the positive rate was 29.7% . And the efflux phenotype positive rate was 31.9 % in carbapenems resistance group, comparing to 13.9 % positive rate in carbapenems sensitive group（x2 = 4.46, P〈0.05）. Conclusion The efflux pumps paly an important role in the resistant mechanism of Acinetobacter Baumannii clinical isolates to carbapenems.

关 键 词：鲍曼不动杆菌 外排泵 耐药 

分 类 号：R446.5[医药卫生—诊断学]