阿尔茨海默病小鼠模型海马组织Atp5a1基因甲基化改变  

Methylation change of Atp5a1 gene in hippocampus Alzheimer's disease mice model

作　　者：唐晓琴 唐红 邓飞[2] 阮思蓓 李昕 陈波 杨朝鲜 熊小明 唐明希 

机构地区：[1]西南医科大学附属医院病理科,四川泸州646000 [2]南方医科大学附属深圳医院病理科,广东深圳518102 [3]西南医科大学神经解剖教研室,四川泸州646000

出　　处：《中国医药科学》2016年第13期24-27,共4页China Medicine And Pharmacy

基　　金：四川省科技厅科技支撑计划(2012SZ0172);四川省教育厅重点项目(16ZA0196);西南医科大学-泸州市科技局联合资助项目[2015LZCYD-S06(6/11)];西南医科大学重点项目(2015-YJ002);西南医科大学附属医院科研项目(15056;15087)

摘　　要：目的初步探讨中期阿尔茨海默病(AD)的presenilin-1/presenilin-2双基因条件性敲除小鼠(d KO mice)模型中海马组织Atp5a1基因的甲基化改变情况。方法运用简化的表观亚硫酸盐测序技术(RRBS)检测3只12月龄雌性d KO mice和3只同系同龄雌性野生型小鼠海马组织基因组DNA异常甲基化情况,利用Bismark(v O.7.4)软件进行对照分析获取异常甲基化基因。结果二代测序结果显示12月龄中度神经退行性病变AD d KO mice海马中Atp5a1基因呈低甲基化状态(P<0.05)。结论 Atp5a1基因在中期AD d KO mice的海马中呈低甲基化状态,提示低甲基化的Atp5a1基因可能作为参与中期AD病程的潜在候选基因。Objective To preliminary discuss methylation change of hippocampus Atp5a1 gene in the mid-term of Alzheimer＇s disease model in mice with presenilin-1/presenilin-2 conditional double knockout. Methods Method of reduced representation bisulphite sequencing（RRBS） was used to screen out the abnormal methylated genes, genomic DNA in hippocampus of three female dKO mice and three female wild-type controls at 12 months of age. Bismark（v O.7.4） software was used to contrast the reference genome sequence. Results The analysis of second generation sequencing showed that Atp5a1 gene in hippocampus of dKO mice at its mid-stage of Alzheimer＇s disease was hypo methylated（P〈0.05）. Conclusion Atp5a1 gene shows hypo methylation status in hippocampus of the dKO mice at 12 months of age. And that indicates that hypo methylated Atp5a1 gene may be a potential candidate gene in the course of mid-term of Alzheimer＇s disease.

关 键 词：Atp5a1基因 阿尔茨海默病 DNA甲基化 dKO MICE 

分 类 号：R34[医药卫生—基础医学]