1型糖尿病小鼠骨髓EPCs的培养和功能研究  

Culture and biological function study of bone marrow endothelial progen-itor cells in type 1 diabetic mice

作　　者：覃媛[1] 蔡轶[1] 张曼[1] 赵莉[1] 

机构地区：[1]广州医科大学药学院蛇毒与生物毒素研究所,广东广州511436

出　　处：《中国医药导报》2016年第22期8-11,F0003,共5页China Medical Herald

基　　金：广东省自然科学基金项目(2016A030310271);广州医科大学科学科研项目(2013C2205)

摘　　要：目的研究1型糖尿病小鼠骨髓内皮祖细胞（EPCs）的培养方法和血管原性功能。方法采用7～8周雄性C57／B6小鼠，腹腔注射链脲佐菌素建立1型糖尿病小鼠模型，同时设立正常对照组，连续5d给等量的柠檬酸缓冲液。采用密度梯度离心法分离并培养糖尿病小鼠骨髓EPcs；通过MTT法、Matrigel、改良Boyden小室分别检测EPCs的增殖、迁移和小管形成的功能性指标。结果与正常对照组比较，培养获得1型糖尿病小鼠的EPCs增殖减少，其迁移和小管形成能力明显下降（P〈0．05）。结论成功培养1型糖尿病小鼠骨髓EPCs，EPCs的血管原功能受损。Objective To study the culture and biological function of bone marrow endothelial progenitor cells （EPCs） in type 1 diabetic mice. Methods C57/B6 male mice with 7-8 weeks old were intraperitoneal injected Streptozotocin to establish type 1 diabetic mice model. While normal control group was set up, and was given the same amount of citric acid buffer for 5 day. EPCs were isolated and cultured by density gradient method from diabetic mice. EPC proliferation were evaluated by using the MTF colorimetric assay. EPC migration was measured by transwell method. EPC tube formation ability was estimated by Matrigel. Results Compared with control mice, the proliferation, migration and tube formation of diabetic EPCs were deceased （P 〈 0.05）. Conclusion Bone marrow endothelial progenitor cells in type 1 diabetic mice were successfully cultured, and the function of diabetic EPCs was impaired.

关 键 词：1型糖尿病 内皮祖细胞 增殖 迁移 小管形成 

分 类 号：R587.1[医药卫生—内分泌]