香蕉α-1,4-葡聚糖淀粉磷酸化酶基因家族全基因组鉴定与进化分析  

Genome-wide Identification and Phylogenetic Analysis of α-1,4-glucan Starch Phosphorylase Gene Family of Banana

作　　者：董晨[1] 胡会刚[1] 贾利强[1] 决登伟[1] 赵秋芳[1] 陈宏良[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业部热带果树分子生物学重点实验室,广东湛江524091

出　　处：《湖北农业科学》2016年第12期3200-3204,共5页Hubei Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务专项(1630062014010);海南省自然科学基金项目(20153134)

摘　　要：为了研究香蕉(Musa nana Lour.)α-1,4-葡聚糖淀粉磷酸化酶(α-1,4-glucan starch phosphorylase,PHS)基因家族的特征和功能,利用生物信息学方法对香蕉PHS家族进行了全基因组查找、鉴定及相关分析。结果表明,在香蕉中共鉴定出2个成员,Ma PHS1的开放阅读框长度为2 784 bp,编码927个氨基酸,分子质量为104.8 ku,等电点为6.59;Ma PHS2的开放阅读框长度为2 517 bp,编码838个氨基酸,分子质量为95.09 ku,等电点为6.09。Ma PHS1和Ma PHS2分别含有17个和15个外显子;Ma PHS1和Ma PHS2均含有淀粉磷酸化酶的保守性基序,进化过程中比较保守。In order to uncover the characteristics and functions of PHS（α-1,4-glucan starch phosphorylase） gene family in banana,the PHS family in banana were searched,identified and analyzed in whole genome level through using the bioinformatics methods. The results showed that two members in banana were identified. The open reading frames of Ma PHS1 and Ma PHS2 were 2 784 and 2 517 bp in length,coded 927 and 838 amino acids with protein molecular weights were 104.8 and95.09 ku, p I were 6.59 and 6.09. Intron / exon structure analysis indicated that Ma PHS1 contained 17 extons and 16 introns,whereas Ma PHS1 contained 15 extons and 14 intron. Ma PHS1 and Ma PHS2 were contained starch phosphorylase conserved motif,Ma PHS were an evolutionarily conserved gene family.

关 键 词：香蕉(Musa nana Lour.) α-1 4-葡聚糖淀粉磷酸化酶 生物信息 基因家族 进化分析 

分 类 号：S668.1[农业科学—果树学]