硝化抑制剂对农业废物好氧堆肥理化性质及反硝化功能基因的影响  

Effect of nitrification inhibitor on physico-chemical properties and denitrification functional genes during agricultural waste composting

作　　者：陈耀宁[1,2] 黄爱知[1,2] 黎媛萍[1,2,3] 黄静霞[1,2] 张嘉超[4] 张洁[1,2] 谭雪斌 马騻 曾光明[1,2] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082 [2]环境生物与控制教育部重点实验室(湖南大学),长沙410082 [3]湖南城市学院化学与环境工程学院,益阳413000 [4]湖南农业大学资源环境学院,长沙410128

出　　处：《环境工程学报》2016年第8期4451-4456,共6页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51378190;21276069);湖南省自然科学基金资助项目(13JJB002);中央高校基本科研业务费专项资金资助

摘　　要：以秸秆等农业废物为主要原料,添加硝化抑制剂双氰胺(dicyandiamide,DCD)进行静态好氧堆肥实验,设置相同堆肥条件不添加DCD作为空白对照,分析了堆肥过程中各个时期不同处理的p H值、NH+4-N浓度、NO-3-N浓度和水溶性有机碳(WSC)理化参数的变化趋势,并应用定量聚合酶链式反应(real-time PCR)技术对参与反硝化过程的功能基因(nir K、nir S和nos Z)丰度随时间的变化情况进行了研究。结果表明,添加DCD明显地改变了堆肥过程的氮素转化,两组堆肥样品的p H值、NH+4-N浓度、NO-3-N浓度有显著差异(p<0.001),但是对WSC的下降过程没有明显影响。DCD对nir K基因的抑制效果最显著,同时nir S和nos Z基因因为理化参数的改变而受到了间接影响。Static aerobic composts based on straw were mixed with the nitrification inhibitor,dicyandiamide. Two tests were set up,one each with and without dicyandiamide. The trends in p H,NH＋4-N,NO-3-N,and water soluble carbon（ WSC） concentrations during composting were analyzed,and the abundances of the denitrification functional genes（ nir K,nir S,and nos Z） were determined using the real-time polymerase chain reaction（ real-time PCR）. The results showed that dicyandiamide had a significant effect on nitrogen transformation in composting. The p H,NH＋4-N,and NO-3-N were influenced significantly by dicyandiamide（ p 0. 001）,but there was no significant effect on WSC during composting. Dicyandiamide significantly inhibited the nir K gene. The nir S and nos Z genes were affected indirectly by the changes in the physicochemical parameters.

关 键 词：好氧堆肥 硝化抑制剂 理化性质 反硝化功能基因 

分 类 号：X705[环境科学与工程—环境工程]