类风湿关节炎患者外周血滤泡辅助性T细胞的表达  

作　　者：祝骥[1] 董浙清[1] 许颖龄[2] 卢德赵[2] 

机构地区：[1]浙江中医药大学附属第三医院检验科,310005 [2]浙江中医药大学生命科学学院,310053

出　　处：《浙江临床医学》2016年第9期1694-1695,共2页Zhejiang Clinical Medical Journal

基　　金：浙江省中医药科技计划（2013ZA066）;浙江中医药大学附属第三医院医药卫生科研计划（ZS13CA01）

摘　　要：目的观察新型cD4＋T辅助细胞亚群．滤泡辅助性T细胞（Tfh）在类风湿关节炎（RA）患者中的表达，探究其在RA发病中的作用及机制。方法采用流式细胞术（FCM）检测RA患者和健康者外周血单个核细胞（PBMC）中Tfh细胞的比例，同时提取淋巴细胞RNA，用实时荧光定量PCR检测Tfh的特异性转录因子Bcl-6，ELISA检测效应分子IL-21。结果与对照组比较，RA患者Tfh细胞的比例明显上调．其特异性转录因子Bcl-6和效应分子IL-21的表达水平均显著上升。结论Tm细胞亚群的功能调节失常可能是RA的重要发病机制之一。Objective To identify the possible roles of a new CD4＋T helper cell subset, T follicular helper cells （ TFH ） , in the pathogenesis of Rheumatoid Arthritis. Methods The percentages of TFH cells in peripheral blood mononuclear cells （ PBMCs ） from patients with RA and healthy controls were assessed by flow cytometry （ FCM ） and then compared.Meanwhile, the expression of TFH transcription factors and cytokines, including Bcl-6 and IL-21 in lymphocytes, was also examined by real-time PCR and ELISA. Results In the patients of RA, the percentage of TFH cells was significantly increased. So did the expression of Bcl-6 and IL-21, the typical transcriptional factor and eytokine of TFH. Our data suggest deregulation of TFH subset in the pathogenesis of RA. The underlying mechanisms remain to be determined. Conclusion Dysfunction of TFH cell subsets may be one of the important pathogenesis of RA.

关 键 词：类风湿关节炎 滤泡辅助性T细胞 流式细胞术 

分 类 号：R512.62[医药卫生—内科学]