MicroRNA-126和血管内皮生长因子在胚胎停育绒毛组织中的表达及相关性研究  

Expressions of microRNA-126 and VEGF in chorionic villi of growth-arrest early embryo and their correlation

作　　者：彭燕[1] 莫吉祥[1] 王新[2] 

机构地区：[1]湘潭职业技术学院妇产科教研室,湖南湘潭411100 [2]中南大学湘雅二医院妇产科,湖南长沙410011

出　　处：《中国现代医学杂志》2016年第15期76-80,共5页China Journal of Modern Medicine

基　　金：湖南省教育厅科学研究项目(No:14C1111)

摘　　要：目的通过分析孕早期胚胎停育与正常胚胎绒毛组织中microRNA-126(miR-126)、血管内皮生长因子(VEGF)的表达差异,探讨其在早期胚胎停育中的相关性。方法收集孕早期胚胎停育和正常胚胎绒毛组织各28例作为胚胎停育组和对照组,采用逆转录-聚合酶链反应(RT-PCR)检测两组miR-126和VEGF mRNA表达水平的变化。结果 1两组绒毛组织中均有miR-126表达,胚胎停育组绒毛组织中miR-126的表达高于对照组,但差异无统计学意义。2胚胎停育组绒毛组织中VEGF的表达低于对照组,差异有统计学意义。3胚胎停育组绒毛组织中miR-126与VEGF的表达呈负相关;对照组绒毛组织中miR-126与VEGF的表达呈正相关。结论 1胚胎停育绒毛组织中VEGF的表达低于正常胚胎。2VEGF的表达变化与胚胎停育的发生有一定的相关性。Objective To investigate the expressions of mieroRNA-126 （miR-126） and vascular endothelial growth factor （VEGF） in the chorionic villi of growth-arrest early embryo group and the ohorionic villus of normal control group, and analyze the different expressions of miR-126 and VEGF between them so as to explore their relationship in early embryo growth arrest. Methods The ehorionic villi of 28 cases in early embryo growth arrest group and 28 cases in control group were collected. Using reverse transcription-poly- mcrase chain reaction （RT-PCR）, the expression levels of miR-126 and IrEGF mRNA in the two groups were detected. Results miR-126 was expressed in the chorionic villi of the early embryo growth arrest group and the control group. The expression of miR-126 in the ehorionic villi of the early embryo growth an＇est group was higher than that of the control group, but the difference was not significant. The expression of VEGF in the chorionic villi of the early embryo growth arrest group was significantly lower than that of the control group, the difference was statistically significant. A negative correlation was found between miR-126 and VEGF in the chorionic villi of the early embryo growth arrest group, while a positive correlation was found between miR-126 and VEGF in the chorionic villi of the control group. Conclusions The expression of VEGF in the chorionie villi of growth-arrest early embryo is lower than that in normal chorionic villi. There is a certain correlation between the change of VEGF expression and the early embryo growth arrest.

关 键 词：胚胎停育 绒毛组织 micro RNA-126 血管内皮生长因子 

分 类 号：R715.3[医药卫生—妇产科学]