利用HMW-GS全缺失突变体快速构建Glu-1位点近等渗入系  

Rapid Development of Glu-1 Locus Near-isogenic Introgression Lines Using HMW-GS Deletion Mutant

作　　者：张星星[1,2] 王召军[2,3] 杨玉双[2,4] 王道文[2] 郑文明[1] 董振营[2] 

机构地区：[1]河南农业大学生命科学学院/小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心,河南郑州450002 [2]中国科学院遗传与发育生物学研究所/植物细胞与染色体工程国家重点实验室,北京100101 [3]中国科学院大学,北京100049 [4]中国热带农业科学院橡胶研究所,海南儋州571731

出　　处：《作物学报》2016年第8期1247-1252,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31300280);国家重点基础研究发展计划(973计划)项目(2013CB127702)资助~~

摘　　要：小麦高分子量麦谷蛋白亚基(high molecular weight glutenin subunit,HMW-GS)由Glu-A1、Glu-B1、Glu-D1位点中含有的复等位基因编码,评价和优化Glu-1位点组合是认识与改良HMW-GS表达与功能的重要途径。本研究创制了以小偃81为背景的HMW-GS基因完全缺失突变体DLGlu1。将DLGlu1与加拿大优质强筋小麦品种Glenlea杂交,结合后代幼胚培养与分子标记辅助选择技术,在BC3F3种子中快速鉴定出来自Glenlea的Glu-A1a、Glu-B1al和Glu-D1d位点不同组合的7种渗入系材料,可进一步发展成一套完整的Glu-1位点有差异的近等渗入系。本研究表明,DLGlu1可用于Glu-1位点近等渗入系的快速创制,对Glu-1位点功能研究和改良具有重要价值。Wheat（Triticum aestivum L., AABBDD） high molecular weight glutenin subunits（HMW-GS） were encoded by the genes located in Glu-A1, Glu-B1, and Glu-D1 loci. Evaluation and optimization of the combination of HMW-GS are very important to understand Glu-1 functions. In this study, we constructed a HMW-GS deletion mutant, DLGlu1 with Xiaoyan 81 background, and crossed it with Glenlea, a Canada elite wheat variety with superior end-use quality. Combining the technologies of wheat embryo culture and molecular marker-assisted selection（MAS）, we obtained seven introgression lines containing Glenlea Glu-A1 a, Glu-B1 al, and Glu-D1 d loci, which can be developed as a complete set of near-isogenic introgression lines possessing Glenlea different HMW-GS genes. Our study indicated that the Glu-1 deletion mutant DLGlu1 is of great value in the fast development of Glu-1 near-isogenic introgression lines and the study and utility of wheat Glu-1.

关 键 词：小麦 高分子量麦谷蛋白亚基 缺失突变体 渗入系 

分 类 号：S512.1[农业科学—作物学]