小麦wzy2-1基因的克隆及功能分析  

Cloning and Functional Analysis of wzy2-1 Gene in Wheat

作　　者：强治全 梁雅珺 于正阳[1] 杜娅[1] 张帅[1] 朱维宁[2] 张林生[1] 

机构地区：[1]西北农林科技大学生命科学学院/旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北大学生命科学学院,陕西西安710069

出　　处：《作物学报》2016年第8期1253-1258,共6页Acta Agronomica Sinica

基　　金：高等学校博士学科点专项科研基金项目(20120204110033);旱区作物逆境生物学国家重点实验室基金项目(CSBAA2015007)资助~~

摘　　要：脱水素是一类植物胚胎发育后期丰富蛋白(late embryogenesis abundant protein,LEA蛋白),属于LEA D-11家族,植物受非生物胁迫会大量表达。利用同源克隆技术,从郑引1号小麦中克隆了1个Kn型脱水素基因,命名为wzy2-1。该基因全长1740 bp,编码579个氨基酸,含有9个保守的K片段,与大麦的Dhn5基因具有较高同源性。生物信息学预测该蛋白属于高度亲水性的无序蛋白。通过该蛋白对大肠杆菌的保护作用研究表明,WZY2-1蛋白能够提高大肠杆菌对低温、高温、高盐以及高渗胁迫的耐受性。荧光实时定量PCR分析表明,wzy2-1基因受低温、盐渍、干旱诱导表达,但不受外源ABA诱导,说明wzy2-1基因属于非ABA依赖型脱水素基因。Dehydrins（DHNs） are identified as the group II of LEA proteins and involved in plant abiotic stress tolerance. In this study, we isolated a novel Kn-type dehydrin gene from wheat cultivar Zhengyin 1, which was designated wzy2-1. The full length of wzy2-1 is 1740 bp, encoding 579 amino acids and containing nine conserved K-fragments. Sequence alignment indicated that wzy2-1 had high homology to Dhn5 gene in Hordeum vulgare. The WZY2-1 protein was predicted to be a highly-hydrophilic and disordered protein. The WZY2-1 protein was successfully expressed in E. coli strain BL21（DE3）. We found that WZY2-1 protein improved the tolerance to low or high temperature, salt and osmotic stresses in E. coli. The q RT-PCR assay indicated that the expression of wzy2-1 gene was induced by low temperature, PEG, and salt stresses rather than ABA. Thus, we conclude that wzy2-1 is an ABA-independent gene.

关 键 词：小麦 脱水素 荧光实时定量PCR 原核表达 

分 类 号：S512.1[农业科学—作物学] Q943.2[生物学—植物学]