利用9个微卫星标记分析海门山羊和徐淮山羊的遗传多样性  

Analyzing Genetic Diversity of Haimen Goat, Xu Huai Goat Based on 9 Microsatellite Makers

作　　者：吕晓阳[1] 孙伟[1] 张想想[1] 高雯[1] 李拥军[1] 张争劲 胡勇[2] 周洪[2] 徐厚生 花卫华 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]徐州市睢宁县林牧渔业局,江苏睢宁221200 [3]徐州华扬羊业有限公司,江苏睢宁221200 [4]镇江市万山红遍农业园区,江苏句容212400

出　　处：《中国畜牧杂志》2016年第15期5-11,共7页Chinese Journal of Animal Science

基　　金：科技部家养动物种质资源平台;国家星火计划重点项目(2012GA690003);江苏省苏北科技计划项目(BN2014003;BN2015013;BN2015014);江苏省农业科技自主创新项目(CX(14)2073);江苏高校优势学科建设工程资助项目;江苏省研究生培养创新工程项目(KYLX16_1402);江苏省工程技术研究中心项目(BM2012308);江苏省六大高峰人才项目

摘　　要：本研究以海门山羊和徐淮山羊为研究对象,应用微卫星标记技术检测这2个山羊品种的遗传结构,并通过与其他地方山羊品种的比较研究,分析了9个微卫星位点(DRBP1、SPS113、OARAE54、MAF209、SRCRSP3、ILSTS087、MAF065、SRCRSP8、ETH10)在这2个品种群体内的遗传变异,包括多态信息含量(PIC)、等位基因频率(AF)、群体杂合度(H)、有效等位基因数(Ne)、等位基因丰度(AR)等。结果表明:所选择的9种微卫星标记在这2种山羊群体上没有表现丰富的多态信息含量,而且变异程度不高。其中,海门山羊、徐淮山羊的平均有效等位基因数分别为2.5179、2.5106;平均多态信息含量分别为0.3110、0.4115;平均等位基因丰度为3.2222、3.5556。海门山羊和徐淮山羊遗传多样性目前不够丰富,急需做好品种遗传资源保护和纯种群体扩繁和提纯复壮恢复工作。In this study, Haimen goat＇ s, Xuhuai goat＇s individuals and 9 microsatellite marker （DRBP1, SPSll3, OARAE54, MAF209, SRCRSP3, ILSTS087, MAF065, SRCRSP8, ETH10） were used to analyze genetic variation of two goat breeds. The genetic variation between populations of these two breeds were analyzed by computing polymorphism information eontent（PIC）, allele frequency（AF）, heterozygosity（H）, effective numbers of alleles（Ne）, allele abundance and compared with other local goat breeds. The results showed that the selected 9 microsatellite markers didn＇t show rich polymorphism information content and the variation degree was not high. Meanwhile, the mean effective numbers of alleles of Haimen goats and Xuhuai goats were 2.5179 and 2.5179, respectively and the average polymorphism information content are 0.3110 and 0.4115, respectively, and the average abundance of alleles are 3.2222 and 3.5556, respectively. According to the research, the conclusion can be reached that currently the genetic diversities of Haimen goats and Xuhuai goats are far from abundance, which urges us to conserve the genetic resources of species, propagate the pedigree groups and improve the purification, rejuvenation and recovery of species.

关 键 词：海门山羊 徐淮山羊 微卫星标记 遗传多样性 种质资源评价 

分 类 号：S827.2[农业科学—畜牧学]