软骨脱细胞基质-Ⅱ型胶原制备组织工程软骨纳米支架的实验研究  

The study on preparation of nanofiber scaffolds with CAEM-COL Ⅱ for cartilage tissue engineering

作　　者：蒋婷[1] 杨泽龙[2] 李小兵[1] 冯刚[3] 

机构地区：[1]川北医学院第二临床医学院.南充市中心医院烧伤整形美容外科,四川南充637000 [2]川北医学院第二临床医学院.南充市中心医院骨科,四川南充637000 [3]川北医学院第二临床医学院.南充市中心医院组织工程与干细胞研究所,四川南充637000

出　　处：《西部医学》2016年第8期1056-1059,1064,共5页Medical Journal of West China

基　　金：国家自然科学基金青年基金(81301568);川北医学院博士科研启动基金(CBY15-QD03)

摘　　要：目的探索软骨脱细胞基质CAEM-Ⅱ型胶原(COLⅡ)通过静电纺丝法制备组织工程纳米支架的可行性。方法将兔肋软骨脱细胞、脱脂、酶解后干燥获得CAEM,再将CAEM和COLⅡ按质量比1∶2的比例混合,通过静电纺丝技术制备组织工程纳米支架,通过测定其吸水率、降解率等检测支架的理化性能,用CCK8法评价其细胞毒性及粘附性情况。结果 CAEM-COLⅡ纳米支架纤维直径为(627±165.4)nm,吸水率为(623.0±27.4)%,35天降解率为(45.6±5.8)%,CCK8法检测结果显示CAEM-COLⅡ复合支架对软骨细胞具有良好的粘附性,生物学性能良好。结论CAEM-COLⅡ纳米支架能为软骨细胞的生长和增殖提供优良的微环境,在组织工程软骨重建中具有潜在的应用价值。Objective To explore the feasibility of preparation of nanofiber scaffolds by electrostatic spinning with cartilage acellular extracellular matrix （CAEM） and collagen Ⅱ（COL Ⅱ） for cartilage tissue engineering. Methods CAEM was obtained from the rabbit rib cartilage after removing the chondrocytes, degreasing, enzymolysis and drying. CAEM and COL Ⅱ were Mixed according to the ratio of 1 ： 2. The nanofiber scaffolds were prepared by electrostatic spinning for cartilage tissue engineering. The physical-chemical properties of the scaffolds were detected through mensurating the porosity, water absorption rate, and degradation rate. The cytotoxicity and cell adhesion were evaluated by CCK8 test. Results The diameter of the CAEM-COLⅡ nanofiber scaffolds was 627nm±165.4nm. The water absorption rate was 623.0%±27.4%, and the degradation rate was 45.6%±5.8% after 35 days. CCK8 test showed that the CAEM- COLⅡ blend scaffolds had good cell adhesion and physical-chemical properties. Conclusion The CAEM COLⅡ nanofiber scaffolds could provide fine microenvironment for the growth and proliferation of chondrocyte. It had potential application value in cartilage tissue engineering.

关 键 词：组织工程纳米支架 Ⅱ型胶原 软骨脱细胞基质 静电纺丝 

分 类 号：R318.08[医药卫生—生物医学工程]