前列腺素E2对CD4^+CD25^+调节性T细胞免疫功能影响的实验研究  

Effect of prostaglandin E2 on the immune function of CD4^+CD25^+ regulatory T cells

作　　者：汤鲁明[1] 王林霞[2] 潘小东[1] 孙来芳[1] 

机构地区：[1]温州医科大学附属第二医院急诊ICU,浙江温州325000 [2]温州医科大学附属第二医院儿童ICU

出　　处：《中华全科医学》2016年第10期1638-1640,1768,共4页Chinese Journal of General Practice

基　　金：浙江省医药卫生科技计划项目(2013RCA039)

摘　　要：目的探讨前列腺素E2对CD4^+CD25^+调节性T细胞免疫功能的影响。方法分离正常BALB/c小鼠脾脏CD4^+CD25^+Treg,利用固相包被抗CD3和加入可溶性CD28辅助活化,予以PGE2刺激。根据刺激剂量不同,分成对照组、A(7μmol/L)、B(14μmol/L)、C(28μmol/L)组,观察PGE2对Treg的增殖、IL-10、IL-2分泌和Foxp3表达的影响。结果 PGE2刺激12 h,3组细胞较对照组增殖反应差异均无统计学意义(P>0.05);B、C组IL-2表达显著降低(P<0.05),而Foxp3表达明显升高(P<0.05);C组IL-10分泌升高(P<0.05);刺激24 h,B、C组细胞增殖反应较对照组显著增强(P<0.05);A、B、C组IL-2分泌均下降(P<0.05),而Foxp3表达均升高(P<0.05);C组IL-10分泌升高(P<0.05);刺激72 h,3组增殖反应、IL-10以及Foxp3表达均显著升高(P<0.05),而IL-2分泌下降(P<0.05)。结论 PGE2能直接增强CD4^+CD25^+Treg的免疫抑制功能,从而可能进一步对效应T细胞免疫功能产生影响。Objective To explore the influence of prostaglandin E2 on the immune function of CD4^＋CD25^＋ regulatory T cells. Methods CD4^＋CD25^＋ Tregs isolated from the spleens of male BALB/C mice were seeded on 96-well cell culture plates coated with anti-CD3 and soluble anti-CD28, and cells were stimulated with prostaglandin E2. According to the different concentrations of PGE2 stimulation, the cells were divided into four groups ： control, A （ 7 μmol/L） , B （ 14 μmol/ L） ,and C（28 μmol/L） groups. The proliferation of cells, IL-10, IL-2 and expression of Foxp3 in Tregs were determined at various intervals. Results After being stimulated with PGE2 for 12 h, the proliferation of cells in A, B, and C groups had no significant difference（P 〉 0.05 ） ,and IL-2, Foxp3 expression in B, C groups were respectively down-regulated and up-regulated markedly（ P 〈 0.05 ）, and IL-10 levels in C group was markedly up-regulated （ P 〈 0.05 ） compared with control group. For 24 h,the proliferation of cells in B, C groups were significantly increased（P 〈 0.05 ） ,IL-2 levels in A, B, and C groups were significantly down-regulated（ P 〈 0.05 ）, but Foxp3 expression was increased （ P 〈 0.05 ）, IL-10 levels in C group was markedly up-regulated（P 〈 0.05 ）. For 72 h, the proliferation of cells, IL-10 and Foxp3 expression in A, B, and C groups was markedly up-regulated （P 〈 0.05 ）, but IL-2 levels in three groups were significantly down-regulated （ P 〈 0.05 ）. Conclusion These data indicate that PGE2 stimulation can directly strengthen immunosuppression function of Treg in vitro, which may further exert some regulating effect on the immune function of effector T cells.

关 键 词：前列腺素E2 调节性T细胞 免疫功能 叉头翼状螺旋转录因子 

分 类 号：R392.12[医药卫生—免疫学] R393[医药卫生—基础医学]