木犀草素抗肿瘤活性研究  

Study of Luteolin on Anti-tumor Effect

作　　者：王月华[1] 李爱峰[2] 付崇罗[1] 张丽[1] 刘红英[1] 玄红专[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]聊城大学化学与化工学院,山东聊城252059

出　　处：《时珍国医国药》2016年第7期1587-1590,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31201860);山东省自然科学基金(No.ZR2012CQ003;No.ZR2014BQ023)

摘　　要：目的研究木犀草素对不同肿瘤细胞的细胞毒性。方法正常培养的肺腺癌细胞(A549)、乳腺癌细胞(MCF-7,MDA-MB-231)及血管内皮细胞(HUVECs)经不同浓度的木犀草素(20,40,80,160μM)分别处理24和48 h,倒置显微镜观察细胞形态,SRB法检测细胞存活率;吖啶橙染色观察细胞核凝集和片段化;Hoechst 33258检测细胞凋亡;荧光探针DCHF检测细胞内活性氧(ROS);Western blotting检测procaspase-3和LC3B的表达。结果木犀草素以剂量依赖的方式抑制肿瘤细胞增殖,上调MDA-MB-231、MCF-7、A549细胞内的ROS和自噬特异标志物LC3-Ⅱ的水平,活化caspase-3的表达。结论木犀草素可能通过诱导凋亡和自噬抑制肿瘤细胞增殖。Objective To study the antitumor activities of luteolin. Methods The normal cultured human lung carcinoma（ A549）,human breast cancer（ MCF- 7,MDA- MB- 231） and human umbilical vein endothelial cells（ HUVECs） were treated with different concentrations of luteolin（ 20,40,80,160 μM） for 24,respectively. The cell viability was measured by SRB method. Hoechst 33258 staining and acridine orange staining were used to observe apoptotic morphology and nuclear fragmentation,respectively. Intracellular reactive oxygen species（ ROS） level was determined by utilizing a fluorescent probe,2＇,7＇- dichlorodihydrofluorescin（ DCHF）. The levels of procaspase3,LC3 B were tested by western blotting. Results Luteolin significantly inhibited proliferation of different cancer cells in a time- and dose- dependent manner and activated caspase 3 level and promoted ROS production. Furthermore,Luteolin also obviously upregulated LC3- Ⅱlevel. Conclusion Luteolin is a potential antitumor agent by inducing apoptosis and autophagy.

关 键 词：木犀草素 抗肿瘤 细胞凋亡 活性氧 

分 类 号：R285.6[医药卫生—中药学]