蚕豆抗绿豆象种质资源的鉴定  

Investigation on Faba Bean Germplasm Resistance to Callosobruchus chinensis L.

作　　者：张红岩[1] 杨涛[1] 关建平[1] 杨生华 方俐[1] 杜萌莹 宗绪晓[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]甘肃省临夏州农业科学院,甘肃临夏731100

出　　处：《作物杂志》2016年第4期86-92,共7页Crops

基　　金：中国农业科学院科技创新工程/作物种质资源鉴定与发掘团队;科技部科技支撑项目(2013BAD01B03-18);农业部作物种质资源保护与利用专项(2015NWB030-09)

摘　　要：仓储型害虫绿豆象对蚕豆的安全储藏和品质经常造成重大损失,严重制约着我国蚕豆产业的发展。选育和利用抗虫品种是防治绿豆象最为安全、有效的途径。在室温条件下,对500份蚕豆资源进行连续两年的绿豆象饱和侵染处理,以期筛选出有利用价值的抗绿豆象种质资源,为蚕豆抗虫性种质创新利用提供依据。筛选结果显示,来源于春播区和秋播区的蚕豆资源,每粒蚕豆虫孔数分别为0.92±0.59、1.05±0.42,差异显著(P<0.05);粒色为青绿色(绿色、青绿、乳白)和深色(紫色、黑色、褐色等)的蚕豆资源,每粒虫孔数分别为1.30±0.62、0.85±0.43,差异显著(P<0.05)。利用Structure软件和Popgen、MEGA软件分别对500份材料的抗虫性进行群体结构分析和聚类分析,可分为5个与地理分布相关的亚群。同时筛选出H0048、H0092、H0094、H001455、H001471、H001787共6份高抗绿豆象的蚕豆资源。Callosobruchus chinensis L., a post harvesting storage pest,often causes heavy losses and quality damages during the faba bean storage process, which severely limited the industrial development of faba bean.Breeding and utilization of resistant varieties is the most safe and effective way to prevent and control the damage of Callosobruchus chinensis L. to faba bean. Five hundred accessions of faba bean genetic resources were thoroughly infected by feeding Callosobruchus chinensis L. which continued for two years at room temperature in order to evaluate their resistance. The results showed that the average puncture hole numbers（0.92±0.59） per seed of each accession from spring sowing area was significantly（P〈0.05） lower than that（1.05±0.42） from autumn sowing area.The average puncture hole numbers（1.30±0.62） per seed of each genotype of cyan（green, light green, milky） group was significantly（P〈0.05） higher than that（0.85±0.43） of dark（purple,black, brown, flesh red） group.Based on their resistance ability of 500 faba bean accessions,five geographic related subgroups could be defined according to cluster analysis of Structure and MEGA softwares.The faba bean accessions,H0048, H0092, H0094, H001455, H001471 and H001787 had been screened out for their high resistance to Callosobruchus chinensis L..

关 键 词：蚕豆 种质资源 绿豆象 抗虫性筛选 抗虫资源 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]