四个玉米自交系根系内生细菌的分离和鉴定  

Isolation and Identification of Endophytic Bacteria from Roots of Four Inbred Lines in Maize

作　　者：许艳蕊[1] 方志军[2] 卢晓平[1] 穆春华[2] 范秋苹 郝鲁江[1] 

机构地区：[1]齐鲁工业大学山东省微生物工程重点实验室,山东济南250353 [2]山东省农业科学院玉米研究所,山东济南250100

出　　处：《作物杂志》2016年第4期112-117,共6页Crops

基　　金：山东省自然科学基金(ZR2012CM019);山东省科技发展计划(2014GNC111014)

摘　　要：对4个玉米自交系(PH4CV、PH6WC、郑58和昌7-2)根系中的内生细菌进行分离和鉴定,为获得有益微生物资源奠定基础。通过形态学观察、生理生化特征鉴定及16S r DNA基因序列同源性分析和构建系统发育树等方法对分离菌株进行生物学鉴定,以确定其种属。共分离到23株内生细菌,鉴定为6个属,包括芽孢杆菌属(Bacillus sp.)、假单胞菌属(Pseudomonas sp.)、寡养单胞菌属(Stenotrophomonas sp.)、肠杆菌属(Enterobacter sp.)、贪噬菌属(Variovorax sp.)、鞘氨醇杆菌属(Sphingobacterium sp.),其中优势属为芽孢杆菌属。4个玉米自交系内生细菌的种类不同,细菌种类由多到少依次是PH6WC、郑58、PH4CV和昌7-2。通过对不同玉米自交系内生细菌的比较分析,发现内生细菌的种类和数量与玉米基因型相关。The endophytic bacteria strains were isolated and characterized from 4 maize（Zea mays L.） inbred lines（PH4CV,PH6 WC,Zheng 58 and Chang 7-2）,which provided beneficial bacterial strains for further research.On the basis of morphological,physiological,biochemistry and 16 S r DNA sequence homology analysis,23 endophytic bacteria were identified to be Bacillus sp.,Pseudomonas sp.,Stenotrophomonas sp.,Enterobacter sp.,Variovorax sp.and Sphingobacterium sp..Of them,Bacillus sp.was predominant,distributed in 4 maize inbred lines.The comparative analysis of endophytic bacteria in different maize inbred lines indicated that the population number of different bacterial species was varied.And the bacteria abundance was associated with maize genotypes as the following order,PH6WC〉Zheng 58〉PH4CV〉Chang 7-2.These results indicate that entophytic bacteria populations and their abundance are associated with maize genotypes.

关 键 词：玉米根系 内生细菌 16S r DNA 分离鉴定 

分 类 号：S513[农业科学—作物学]