土壤磷酸酶活性测定方法的改进  

Improvement of soil phosphatase activity measurement method

作　　者：石春芳[1,2] 王志勇[3] 冷小云[1] 刘菊梅[1] 曹菊梅 

机构地区：[1]内蒙古科技大学数理与生物工程学院,内蒙古包头014010 [2]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [3]内蒙古包头市土壤肥料工作站,内蒙古包头014010 [4]内蒙古包头北郊污水处理厂,内蒙古包头014010

出　　处：《实验技术与管理》2016年第7期48-49,54,共3页Experimental Technology and Management

基　　金：包头市科技计划项目(2014Z1010-3);内蒙古自治区自然科学基金项目(2015MS0332)

摘　　要：磷酸酶是土壤中非常重要的酶类,其活性可作为评价土壤磷素生物转化方向与强度的指标,快速、简便、准确的测定方法对磷酸酶的研究具有促进作用。在磷酸苯二钠比色法基础上进行改进,用酶标板/酶标仪测定吸光度,不仅减少了试剂使用量,而且缩短了测定时间,在样品量较大时可降低操作人员的工作强度。Phosphatase was very important soil enzymes which has been involved in the conversion of organic phosphorus in the soil,and its activity could be valued as indicator of soil phosphorus biotransformation direction and strength.The fast,simple and accurate measuring method has a facilitating role in phosphatases research.Improvement method is based on Disodium phosphate benzene colorimetric method,and the absorbance was measured by microtiter plates/ microplate.Not only the amount of reagent use has been reduced,but also the measurement time has been shortened,and the operators＇ work intensity can be reduced when there were lots of samples.

关 键 词：土壤 磷酸酶活性 测定方法 

分 类 号：S151.9[农业科学—土壤学]