‘KRS’芒果发育过程中糖积累与代谢相关酶活性的关系  

The Relationship between Sugar Accumulation and Related Enzyme Activities during the Development Process of ‘KRS' Mango Fruits

作　　者：武红霞[1] 姚全胜[1] 王松标[1] 马小卫[1] 詹儒林[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所,农业部热带果树生物学重点实验室,广东湛江524091

出　　处：《安徽农业科学》2016年第17期24-26,55,共4页Journal of Anhui Agricultural Sciences

基　　金：农业行业科研专项子项目(201203092-3);广东省自然科学基金粤东西北创新人才联合培养项目(2016A030307006);科研院所基本科研业务费专项(1630062015001;1630062015007)

摘　　要：[目的]探讨芒果果实糖积累和转化的生理机制。[方法]以‘KRS’芒为试材,研究芒果果实生长发育和成熟过程中的淀粉、蔗糖、葡萄糖和果糖含量变化,与淀粉酶、蔗糖代谢相关酶——酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性的相关性。[结果]果实发育前期‘KRS’主要积累淀粉、葡萄糖和果糖,成熟时淀粉酶活性降至最低,淀粉水解快速积累蔗糖。在整个发育过程中AI活性维持最高,完熟时降低,SPS在果实发育中期略有降低,完熟时升至最高,SS和NI一直很低且较稳定。相关性分析表明淀粉含量与酸性转化酶活性呈显著正相关;蔗糖、葡萄糖含量与SPS、SS呈极显著正相关;果糖含量与SS、AI均呈极显著正相关。[结论]芒果成熟时淀粉分解、AI活性降低和SPS、SS活性的增加是引起‘KRS’果实蔗糖积累的主要因子。Objective] The aim was to discuss physiological mechanism of sugar accumulation and transformation in mango fruit.[Method] The relationship between the contents of starch, sucrose, glucose and fructose, and the activities of amylase, sucrose metabolizing enzymes in-cluding acid invertase （AI）, neutral invertase （NI）, sucrose synthase（SS） and sucrose phosphate synthase（SPS） during fruit development process in KRS mango were studied.[Result] The results showed that starch, fructose and glucose accumulated at the early stage of fruit de-velopment, amylase activity dropped to minimum along with the starch decomposition and sucrose accumulated at the ripening stage .The ac-tivities of AI maintained higher throughout the developmental stage, dropped slightly at the ripening stage.The SPS activities dropped slightly in middle stage of fruit development, then raised to the highest at the ripening stage.The activities of SS and NI maintained minimum and changed very little throughout the development process.The starch content had positive correlation with amylase.The contents of sucrose and glucose had significantly positive correlation with the activities of SPS and SS .The content of fructose had significantly positive correlation with SS and AI.[Conclusion] Decomposition in starch, reduction in AI, increment in SS and SPS activities are responsible for sucrose accumula-tion in developing fruits of mango ‘KRS’ .

关 键 词：芒果 糖积累 转化酶 蔗糖合成酶 蔗糖磷酸合成酶 

分 类 号：S601[农业科学—园艺学]