白蔹多糖的分离纯化及其对小鼠脾细胞增殖的影响  

Separation and Purification of Polysaccharides from Ampelopsis Japonica( Thunb. ) Makino and Its Effects on Proliferation Function of Mice Spleen Lymphocyte

作　　者：李岩[1] 赵宏[1] 王宇亮[1] 初维 张宇[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2016年第17期137-140,共4页Journal of Anhui Agricultural Sciences

基　　金：佳木斯大学校级创新团队(CXTD-2013-05);佳木斯大学青年创新人才培养计划(22Zq201501);佳木斯大学研究生科技创新项目(LZZ2015_030)

摘　　要：[目的]分离纯化白蔹多糖,研究白蔹均一多糖对小鼠脾淋巴细胞增殖的影响。[方法]采用水提醇沉法提取白蔹多糖,通过三氯乙酸法脱除蛋白,用中空纤维超滤膜和DEAE Cellulose DE-52对白蔹多糖进行分离纯化,高效凝胶过滤色谱法鉴定纯度并测定分子量,PMP柱前衍生化测定单糖组成,采用MTT法测定均一多糖对Con A和LPS诱导的小鼠脾淋巴细胞增殖的影响。[结果]PAJM-A均一多糖分子量为1.29×105u,由甘露糖(Man)、鼠李糖(Rha)、葡萄糖醛酸(Glc A)、半乳糖(Gal)、葡萄糖(Glc)、木糖(Xly)组成,其物质的量比为1.36∶1.87∶2.02∶1.58∶1.04,均一多糖对Con A诱导的T淋巴细胞和LPS诱导的B淋巴细胞有较显著的增殖作用。[结论]PAJM-A是一种新的白蔹均一多糖,并与Con A、LPS对T、B淋巴细胞的增殖有协同刺激作用。Objective] To separate and purify polysaccharides from Ampelopsis Japonica （ Thunb.） Makino, and to study its effects on prolif-eration function of mice spleen lymphocyte transformation.[Method] Hot water extracting and TCA method were employed to isolate the PA-JM.Hollow fiber ultrafiltration membrane and the DEAE Cellulose 52 were used to separate and purify polysaccharides, and the monosaccha-ride composition, and structure were preliminarily identified by IR and HPLC.Their effects on proliferation function of mice spleen lymphocyte transformation were determined by MTT.[Result] PAJM-A was 1.29 ×105 u and it was mainly composed of Man, Rha, GlcA, Glc, Gal and Xyl in the molar ratio of 1.36∶1.87∶2.02∶0.10∶1.58∶1.04.And the homogeneous polysaccharides were able to increase the proliferation of lymphocytes induced by ConA and LPS.[ Conclusion] PAJM-A is a novel homogeneous polysaccharide, PAJM-A can effectively increase lymphocyte proliferation and enhance the effect of ConA, LPS-induced T and B cell growth.

关 键 词：白蔹 多糖 分离纯化 脾淋巴细胞 

分 类 号：S567[农业科学—中草药栽培]