Cd胁迫下菠菜叶片微观组织结构变化的测量和定量分析  

Quantitative Analysis on the Changes of Microstructure of Spinach Leaves under the Stress of Cadmium

作　　者：孙春美[1,2] 陈明[1,2,4] 曹晓娟[3] 冯流[1] Jacques Yvon 

机构地区：[1]北京化工大学,北京100029 [2]中国地质科学院矿产资源研究所,北京100037 [3]中国地质环境监测院,北京100081 [4]Laboratoire Environnement et Mineralurgie,15 avenue du Charmois,54500 Nancy France

出　　处：《环境科学与技术》2016年第7期34-39,共6页Environmental Science & Technology

基　　金：科技部国际合作重点项目(2005DFA91140);国土资源部公益性行业基金项目(201211039);国土资源部公益性行业基金项目(201411089)

摘　　要：该文介绍了OCT技术进行Cd胁迫下菠菜叶片微观组织结构变化的测量方法,提出了平均光强、反射能和均一性等3个定量分析新指标。通过研究平均光强、反射能和均一性3个指标随菠菜生长时间和土壤重金属含量的变化,获得了规律性较好的结果:(1)平均光强:上表皮>栅栏组织/海绵组织>下表皮;反射能:上表皮<栅栏组织<海绵组织<下表皮;均一性:上表皮和下表皮较均一,而栅栏组织和海绵组织均一性差。(2)不同的组织层的定量指标具有不同的变化规律,但同一个层位的指标具有较好的规律性。(3)随菠菜生长时间的增加,各组织层的反射光平均光强增大。反射能呈现芽期和成熟期高,生长期较低的规律。(4)随土壤Cd浓度的增加,各组织层的平均光强、反射能和均一性均有一定程度的增大趋势,但不同组织层的具体变化情况有区别。以上规律性成果意味着,利用OCT技术进行重金属的生态效应评价具有现实可能性。The methodology to measure the microstmcture change of vegetables, spinach leaves as an example, under the stress of cadmium via OCT technology is introduced. Three new indicators, including average intensity, reflection energy and uniformity, are suggested to quantitatively assess the ecological effect of heavy metals. Results of bonsai tests and OCT detections show that average intensity values changes as upper epidermis〉 palisade tissue〉 spongy tissue〉lower epidermis. The rules of the quantitative indicators of the tissues layers are different from each other, but those of the same layer are regular. With the increase of the growth time, average intensity is generally increased. The reflection energy is high during the bud and maturity stages, while it is low during the growth period. With the increase of Cd concentration in soil, average intensity, reflection energy and uniformity of each layer increase, however, there are some differences in the specific conditions of different layers. The result also implies that it is possible to assess the ecological effects of heavy metals by means of OCT technology.

关 键 词：重金属 生态效应 微观组织结构 菠菜 镉 定量分析 

分 类 号：X503.231[环境科学与工程—环境工程]