混菌发酵降解茶皂素的条件优化  

Optimum Conditions for Mixed Bacteria Degradation on Tea Saponin

作　　者：黄浦[1,2] 肖瑜[1,2] 覃妍[1,2] 梁美娜[1,2] 唐沈[1,2] 易茗[1,2] 朱芸[1,2] 

机构地区：[1]桂林理工大学广西环境污染控制理论与技术重点实验室 [2]桂林理工大学广西岩溶地区水污染控制与用水安全保障协同创新中心,广西桂林541004

出　　处：《环境科学与技术》2016年第7期100-104,113,共6页Environmental Science & Technology

基　　金：国家自然科学基金(21367010)

摘　　要：以茶皂素为研究对象,以茶皂素的降解率为评价指标,利用地衣芽孢杆菌与铜绿假单胞菌混合发酵降解茶皂素,分别探讨接种比例、发酵时间、发酵温度、摇床转速及接种量对其降解的影响,再通过曲面响应设计实验对发酵条件进行优化。结果表明,影响降解率显著性次序为:发酵时间>发酵温度>接种量>摇床转速>接种比例,得到最佳的优化条件为:混菌接种比例1∶2(V/V),发酵时间12.8 d,发酵温度30.68℃,摇床转速150 r/min,接种量为8.81%,在此条件下进行发酵,茶皂素的降解率达到(73.76±0.63)%。说明混菌对茶皂素的降解有着较明显的效果。Using tea saponin as raw material,with tea saponin degradation rate as the evaluation index,tea saponin was degraded by Bacillus licheniformis and Pseudomonas aeruginosa mixed, fermentation time, fermentation temperature,rotation speed and inoculum size on its degradation were investigated,and through the response surface design experiments the fermentation conditions were optimized. The results show that, the degradation rate was affected in the order as fermentation time fermentation temperature quantity of fermentation rotation speed vaccination ratio. The optimum fermentation degradation conditions were studied as mixed bacteria inoculation ratio of 1 ∶2（V/V）,fermentation time 12.8 d,fermentation temperature 30.68 ℃,rotation speed 150 r/min,inoculated quantity 8.81%,in this condition for fermentation,decomposition rate of tea saponin reached（73.76 ±0.63）%, indicating the mixed bacteria has obvious effect on the degradation of tea saponin.

关 键 词：地衣芽孢杆菌 铜绿假单胞菌 混菌发酵 茶皂素 条件优化 

分 类 号：X50[环境科学与工程—环境工程]