胃转流术对非肥胖型2型糖尿病大鼠骨骼肌胰岛素受体底物-1及泛素化蛋白表达的影响  

Influence on Expressions of Insulin Receptor Substrate 1 and Ubiquitin-Protein in Skeletal Muscle of Non-Obese Rats with Type 2 Diabetes Mellitus Following Gastric Bypass Operation

作　　者：龚帅 任泽强 张蓬波 姚丹 刘利 王吉科 孙婷 

机构地区：[1]徐州医科大学附属医院普外科,江苏徐州221000 [2]徐州医科大学研究生学院,江苏徐州221000

出　　处：《中国普外基础与临床杂志》2016年第8期915-921,共7页Chinese Journal of Bases and Clinics In General Surgery

基　　金：徐州市科技计划资助项目(编号:XM12B028)~~

摘　　要：目的观察胃转流术对非肥胖型2型糖尿病（T2DM）大鼠骨骼肌中胰岛素受体底物1（IRS-1）、泛素化蛋白表达的影响，探讨胃转流术改善骨骼肌胰岛素抵抗的可能机制。方法雄性GK大鼠随机分为糖尿病手术组、糖尿病假手术组及糖尿病对照组（每组8只），另外随机抽取8只Wistar大鼠作为正常对照组。检测术前及术后第1、2、4、8周时各组大鼠空腹体重、空腹血糖（FPG）、空腹胰岛素（FINS）水平，计算各组术前与术后第8周时稳态模式胰岛素抵抗（HOMA—IR）指数。应用Westernbla技术检测各组术后第8周时骨骼肌中IRS-1蛋白和泛素化蛋白的表达水平。结果①术后第1、2、4周时，糖尿病手术组大鼠体重与术前比较明显下降（P〈0．05），而术后第8周时大鼠体重又恢复到术前水平（P〉0．05）；糖尿病假手术组术后第1周时与术前相比下降明显（P〈0．05），而第4周和第8周时又呈上升趋势，与术前相比明显增加（P〈0．05）；糖尿病对照组和正常对照组术后大鼠体重均呈稳步上升趋势，至术后第8周时体重明显高于术前（P〈0．0S）。②术前，糖尿病手术组、糖尿病假手术组及糖尿病对照组FPG均明显高于正常对照组（P〈0．05）；术后第1、2、4、8周时，糖尿病假手术组和糖尿病对照组均明显高于正常对照组（P〈0．05）；术后第2、4、8周时，糖尿病手术组明显低于相应时相糖尿病假手术组（P〈O．05）和糖尿病对照组（P〈0．05），也明显低于术前水平（P〈0．05）；其余3组组内手术前、后比较差异均无统计学意义（P〉0．05）。③术前4组间FINS水平比较差异均无统计学意义（P〉0．05）；糖尿病手术组FINS经历了先升后降的过程，至术后第8周时已明显低于术前及相同时相的糖尿病假手术组和糖尿病对照组（P〈O．05），但与正常对照组问比较差异无统计学意义（PObjective To observe expre with ssions of insulin receptor substrate 1 （IRS-1） and ubiquitin-protein in skeletal muscle of non-obese rats with type 2 diabetes mellitus following gastric bypass operation （GBP）, and to investigate possible mechanism of GBP in improving insulin resistance. Methods Male GK rats were randomly divided into diabetic operation group （DO group）, diabetic sham operation group （DSO group）, and diabetic control group （DC group）, 8 rats in each group; besides 8 male Wistar rats were served as normal control group （NC group）. Fasting body weight （FBW）, fasting plasma glucose （FPG）, and fasting insulin （FINS） were measured respectively before operation and on week 1, 2, 4 and 8 after operation. Homeostasis model-insulin resistant （HOMA-IR） index was calculated respectively before opera- tion and on week 8 after operation. The expressions of IRS-1 protein and ubiquitin-protein in skeletal muscle were detected by using Western blot method on week 8 after operation. Results （1） Compared with the preoperative levels, the FBWs on week 1, 2, and 4 after operation markedly decreased （P〈0.05）, but it recovered to the preoperative level on week 8 after operation （P〉0.05） in the DO group; which in the DSO group decreased on week 1 after operation （P〈0.05） and then increased on week 4 after operation （P〈0.05）; which in the DC group or the NC group increased continuously and had a significant difference on week 8 after operation （P〈0.05）. （2） The FPGs in the DO, DSO and DC groups were significantly higher than those of the NC group before operation （P〈0.05）, which in the DO group decreased from （9.10±0.98） mmoL/L before operation to （5.70±0.91） mmol/L on week 8 after operation （P〈0.05） and were significantly lower than those of the DSO group or the DC group on week 2, 4, and 8 after operation （P〈0.05）; which in the DC group, DSO group and NC group had no obviously changes between before and after ope

关 键 词：胃转流术 2型糖尿病 胰岛素受体底物-1 泛素化蛋白 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌] R656.6[医药卫生—内科学]