KcERF-PeDREB2a双价基因对棉花干旱、盐碱耐受性的影响  

Effect of Bivalent Gene Kc ERF-Pe DREB2a on Drought and Salt Tolerance of Cotton

作　　者：董雪妮[1,2] 高丽华[2] 丁梦琦[1] 周美亮[2] 唐益雄[2] 李金博 邵继荣[1] 吴燕民[2] 

机构地区：[1]四川农业大学生命科学学院,四川雅安625001 [2]中国农业科学院生物技术研究所,北京100081 [3]深圳市铁汉生态环境股份有限公司,广东深圳518000

出　　处：《中国农业科技导报》2016年第4期17-23,共7页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2014ZX08005-004);广东省省级科技计划项目(2015B090904008)资助

摘　　要：利用农杆菌介导法将耐旱耐盐转录因子基因Pe DREB2a和Kc ERF导入受体材料陆地棉R15中,获得12个株系的转基因植株,通过硫酸卡那霉素初筛以及PCR分子检测最终获得7个转Kc ERF-Pe DREB2a基因的棉花株系。通过测定转基因棉花的抗逆相关生理指标以及对基因相对表达量测定分析,结果表明,200mmol/L的Na Cl和15%的PEG-6000胁迫处理后,转基因棉花幼苗的CAT、SOD活性,游离脯氨酸含量均高于对照组,MDA含量较对照组棉花明显下降。实时定量RT-PCR结果表明,干旱胁迫下,转基因棉花幼苗叶片中Pe DREB2a基因的表达量高于Kc ERF基因的表达量;高盐胁迫下,转基因棉花叶片中的Pe DREB2a,Kc ERF基因的相对表达量持平。本研究结果表明,在干旱、高盐胁迫下,Kc ERF-Pe DREB2a基因有助于提高棉花的耐旱耐盐能力。Transcription factor gene PeDREB2a and KcERF were inserted to the upland cotton R15 mediated by agrobacterium and 12 strains of transgenic plants were obtained. Through kanamycin screening and gaolecular testing, 7 generation strains with PeDREB2a and KcERF were obtained. This paper measured the physiological indexes related to stress tolerance and analyzed relative exprsssion of genes, and the results showed that under the stress of 200 mmol/L NaC1 and 15% PEG-6000, the activity of CAT and SOD, free proline content of transgenic cotton seedling were higher than the control, the MDA content significantly lower than control. Real-time PCR results showed that under drought stress, PeDREB2a relative expression in transgenic cotton seedling leaf was higher than KcERF ; under salt stress, PeDREB2a and KcERF relative expression in transgenic cotton was fiat. This suggested that KcERF-PeDREB2a contributed to imorove the droudat and salt tolerance ability in cotton.

关 键 词：农杆菌 转基因棉花 耐旱耐盐 生理指标 相对表达量 

分 类 号：Q786[生物学—分子生物学] S562[农业科学—作物学]