Global versus Chinese perspectives on the phylogeny of the N-fixing clade  

作　　者：Hong-Lei Li Wei Wang Rui-Qi Li Jing-Bo Zhang Miao Sun Rehan Naeem Jun-Xia Su Xiao-Guo Xiang Peter E. Mortimer De-Zhu Li Kevin D. Hyde Jian-Chu Xu Douglas E. Soltis Pamela S. Soltis Jianhua Li Shou-Zhou Zhang Hong Wu Zhi-Duan Chen An-Ming Lu 

机构地区：[1]State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China [2]Shenzhen Key Laboratory of Southern Subtropical Plant Diversity, Fairylake Botanical Garden, Shenzhen and Chinese Academy of Sciences,Shenzhen 518004, Guangdong, China [3]College of Life Sciences, South China Agricultural University, Guangzhou 510642,China [4]College of Life Sciences, Shanxi Normal University, Linfen 041004, Shanxi, China [5]World Agroforestry Centre, East and Central Asia, Kunming 650201, China [6]Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201,China [7]plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming650201, China [8]School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand [9]Department of Biology, University of Florida, Gainesville, FL 32611, USA [10]Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA [11]Genetics Institute, University of Florida, Gainesville, FL 32610, USA [12]Biology Department, Hope College, Holland, MI 49423, USA

出　　处：《Journal of Systematics and Evolution》2016年第4期392-399,共8页植物分类学报（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China （Grant Nos. 31590822, 31500175, 31270268 and 40830209）, the National Basic Research Program of China （Grant No. 2014CB954100）, Chinese Academy of Sciences International Institution Development Program （No. SAJC201315）, Chinese Academy of Sciences External Cooperation Program of the Bureau of Interna- tional Co-operation （No. GJHZ201321）, Chinese Academy of Sciences Visiting Professorship to Douglas E. Soltis as a senior international scientist （Grant No. 2011T1S24）, and Shenzhen Key Laboratory of Southern Subtropical Plant Diversity （Grant No. SSTLAB-2014-04）.

摘　　要：There has been increasing interest in integrating a regional tree of life with community assembly rules in the ecological research. This raises questions regarding the impacts of taxon sampling strategies at the regional versus global scales on the topology. To address this concern, we constructed two trees for the nitrogen-fixing clade： （i） a genus-level global tree including 1023 genera; and （ii） a regional tree comprising 303 genera, with taxon sampling limited to China. We used the supermatrix approach and performed maximum likelihood analyses on combined matK, rbcL, and trnL-F plastid sequences. We found that the topology of the global and the regional tree of the N-fixing clade were generally congruent. However, whereas relationships among the four orders obtained with the global tree agreed with the accepted topology obtained in focused analyses with more genes, the regional topology obtained different relationships, albeit weakly supported. At a finer scale, the phylogenetic position of the family Myricaceae was found to be sensitive to sampling density. We expect that internal support throughout the phylogeny could be improved with denser taxon sampling. The taxon sampling approach （global vs. regional） did not have a major impact on fine-level branching patterns of the N-fixing clade. Thus, a well-resolved phylogeny with relatively dense taxon sampling strategy at the regional scale appears, in this case, to be a good representation of the overall phylogenetic pattern and could be used in ecological research. Otherwise, the regional tree should be adjusted according to the correspondingly reliable global tree.There has been increasing interest in integrating a regional tree of life with community assembly rules in the ecological research. This raises questions regarding the impacts of taxon sampling strategies at the regional versus global scales on the topology. To address this concern, we constructed two trees for the nitrogen-fixing clade： （i） a genus-level global tree including 1023 genera; and （ii） a regional tree comprising 303 genera, with taxon sampling limited to China. We used the supermatrix approach and performed maximum likelihood analyses on combined matK, rbcL, and trnL-F plastid sequences. We found that the topology of the global and the regional tree of the N-fixing clade were generally congruent. However, whereas relationships among the four orders obtained with the global tree agreed with the accepted topology obtained in focused analyses with more genes, the regional topology obtained different relationships, albeit weakly supported. At a finer scale, the phylogenetic position of the family Myricaceae was found to be sensitive to sampling density. We expect that internal support throughout the phylogeny could be improved with denser taxon sampling. The taxon sampling approach （global vs. regional） did not have a major impact on fine-level branching patterns of the N-fixing clade. Thus, a well-resolved phylogeny with relatively dense taxon sampling strategy at the regional scale appears, in this case, to be a good representation of the overall phylogenetic pattern and could be used in ecological research. Otherwise, the regional tree should be adjusted according to the correspondingly reliable global tree.

关 键 词：global tree of life N-fixing clade PHYLOGENY regional tree of life supermatrix. 

分 类 号：Q949[生物学—植物学]