A supermatrix approach provides a comprehensive genus-level phylogeny for Gentianales  

作　　者：Lei-Lei Yang Hong-Lei Li Lei Wei Tuo Yang Dai-Yong Kuang Ming-Hong Li Yi-Ying Liao Zhi-Duan Chen Hong Wu Shou-Zhou Zhang 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China [2]Shenzhen Key Laboratory of Southern Subtropical Plant Diversity, Fairylake Botanical Garden, Shenzhen and Chinese Academy of Sciences, Shenzhen 518004, Guangdong, China [3]State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China [4]Marine Environmental Monitor Center of Shanwei, State Oceanic Administration, Shanwei 516600, Guangdong, China [5]Hengshan National Nature Reserve Administration, Hengshan 421900, Hunan, China

出　　处：《Journal of Systematics and Evolution》2016年第4期400-415,共16页植物分类学报（英文版）

基　　金：This research was supported by The Scientific Research Fund of Fairylake Botanical Garden （the study of deep relationships in Apocynaceae phylogeny）, Shenzhen Key Laboratory of Southern Subtropical Plant Diversity （Grant No. SSTLAB-2o14- o4）, National Key Basic Research Program of China （Grant No. 2014CB954100）, Chinese Academy of Sciences International Institution Development Program （Grant No. SAJC201315）, the Shenzhen Science and Technology Innovation Council Fund （Grant No. KQC 201105310009A）, Chinese Academy of Sciences External Cooperation Program of BIC （Grant No. GJHZ201321）, and the National Natural Science Foundation of China （Grant No. 31500175）.

摘　　要：Gentianales consist of Apocynaceae, Gelsemiaceae, Gentianaceae, Loganiaceae, and Rubiaceae, of which the majority are woody plants in tropical and subtropical areas. Despite extensive efforts in reconstructing the phylogeny of Gentianales based on molecular data, some interfamily and intrafamily relationships remain uncertain. We reconstructed the genus-level phylogeny of Gentianales based on the supermatrix of eight plastid markers （rbcL, marK, atpB, ndhF rp116, rps16, the trnL-trnF region, and atpB-rbcL spacer） and one mitochondrial gene （matR） using maximum likelihood. The major clades and their relationships retrieved in the present study concur with those of previous studies. All of the five families of Gentianales are monophyletic with strong support. We resolved Rubiaceae as sister to the remaining families in Gentianales and showed support for the sister relationship between Loganiaceae and Apocynaceae. Our results provide new insights into relationships among intrafamilial clades. For example, within Rubiaceae we found that Craterispermeae were sister to Morindeae ＋ （Palicoureeae ＋ Psychotrieae） and that Theligoneae were sister to Putorieae. Within Gentianaceae, our phylogeny revealed that Gentianeae were sister to Helieae and Potalieae, and subtribe Lisianthiinae were sister to Potaliinae and Faroinae. Within Loganiaceae, we found Neuburgia as sister to Spigelieae. Within Apocynaceae, our results supported Amsonieae as sister to Melodineae, and Hunterieae as sister to a clade comprising Plumerieae ＋ （Carisseae ＋ APSA）. We also confirmed the monophyly of Perplocoideae and the relationships among Baisseeae ＋ （Secamonoideae ＋ Asclepiadoideae）.Gentianales consist of Apocynaceae, Gelsemiaceae, Gentianaceae, Loganiaceae, and Rubiaceae, of which the majority are woody plants in tropical and subtropical areas. Despite extensive efforts in reconstructing the phylogeny of Gentianales based on molecular data, some interfamily and intrafamily relationships remain uncertain. We reconstructed the genus-level phylogeny of Gentianales based on the supermatrix of eight plastid markers （rbcL, marK, atpB, ndhF rp116, rps16, the trnL-trnF region, and atpB-rbcL spacer） and one mitochondrial gene （matR） using maximum likelihood. The major clades and their relationships retrieved in the present study concur with those of previous studies. All of the five families of Gentianales are monophyletic with strong support. We resolved Rubiaceae as sister to the remaining families in Gentianales and showed support for the sister relationship between Loganiaceae and Apocynaceae. Our results provide new insights into relationships among intrafamilial clades. For example, within Rubiaceae we found that Craterispermeae were sister to Morindeae ＋ （Palicoureeae ＋ Psychotrieae） and that Theligoneae were sister to Putorieae. Within Gentianaceae, our phylogeny revealed that Gentianeae were sister to Helieae and Potalieae, and subtribe Lisianthiinae were sister to Potaliinae and Faroinae. Within Loganiaceae, we found Neuburgia as sister to Spigelieae. Within Apocynaceae, our results supported Amsonieae as sister to Melodineae, and Hunterieae as sister to a clade comprising Plumerieae ＋ （Carisseae ＋ APSA）. We also confirmed the monophyly of Perplocoideae and the relationships among Baisseeae ＋ （Secamonoideae ＋ Asclepiadoideae）.

关 键 词：Gentianales maximum likelihood PHYLOGENY supermatdx approach. 

分 类 号：Q949[生物学—植物学]