HPLC法同时测定玉泉丸中5个成分的含量  

Simultaneous Determination of Five Bioactive Components in Yuquan Pills by RP-HPLC

作　　者：李少光[1] 蔡林雪[2] 姚宏[1] 黄丽英[1] 陈冰[1] 林新华[1] 

机构地区：[1]福建医科大学药学院,福州350801 [2]南京军区福州总医院第四七六医院药剂科,福州350002

出　　处：《福建医科大学学报》2016年第3期155-158,共4页Journal of Fujian Medical University

基　　金：国家863项目(2012AA022604);国家自然科学基金项目(81202987);福建省高校新世纪优秀人才支持计划(JA14128);福建省科技厅自然科学基金项目(2016J01368);福建省教育厅科技项目(JA15209);福建医科大学苗圃科研基金(2014MP010)

摘　　要：目的 建立同时测定玉泉丸中5个成分（葛根素、甘草苷、异甘草苷、大豆苷元和甘草酸）的方法。方法 采用反相高效液相色谱-二极管阵列紫外检测器（RP-HPLC-DAD）,在UltimateTM XB-C18色谱柱（250×4.6mm,5μm）上,优化获得以乙腈（B）-水（0.2%甲酸）（A）为流动相的梯度洗脱条件;流速1.0mL/min;检测波长为254,280和340nm,柱温30℃。结果 5个活性成分的分离度良好,在各自测定的浓度范围内具有良好的线性关系（r≥0.997）,平均加样回收率为87.5%-104.3%。相对标准偏差≤3.3%（n=6）。结论 本方法操作简便、快速,具有较好的稳定性和重复性,可为玉泉丸质量控制提供定量分析方法。Objective To establish a quality control method by using reversed-phase high performance liquid chromatography coupled with DAD detector （RP-HPLC-DAD） to simultaneously determine five components （puerarin, liquirtin, isoliquiritin, daidzein, glycyrrhizic acid） in Yuquan pills. Method Chromatographic experiment was carried out on an UltimateTM XB-C18 column（250 × 4.6 mm, 5 μm）with a gradient elution composed of acetonitrile （B） and 0.2% formic acid（A）. The flow rate was 1.0 mL/min and the detection wavelength were 254, 280, 340 nm. The column temperature was set at 30 ℃. Results The calibration curves of five components （puerarin, liquirtin, isoliquiritin, daidzein, glycyrrhizic acid） showed good linearity in their concentration ranges with r;0. 997. The average recovery was 87.5 % - 104.3 % with relative standard deviation （RSD） less than 3.3% （n = 6）. Conclusion The method is rapid, simple, and with good repeatability and stability, and it can be used in quality control of Yuquan pills.

关 键 词：玉泉丸 葛根素 甘草 异黄酮类 甘草酸 色谱法 高压液相 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]