橡胶防老剂4020、4010NA对人胚肺成纤维细胞的毒性对比  

A comparative study of toxicity of rubber antioxidant 4020 and 4010NA on human embryonic lung fibroblast cells

作　　者：朱勇[1] 丁璐[2] 徐艳琼[1] 

机构地区：[1]江苏省疾病预防控制中心职业病防治所,江苏南京210028 [2]苏州市疾病预防控制中心职业放射卫生科,江苏苏州215000

出　　处：《职业与健康》2016年第14期1991-1992,1996,共3页Occupation and Health

基　　金：江苏预防医学项目(Y2012090)

摘　　要：目的初步探讨橡胶防老剂4020和4010NA对人胚肺成纤维细胞(HELF)的毒性差异和剂量-反应趋势。方法橡胶防老剂4020和4010NA以终浓度3、6、9、12μg/ml处理HELF细胞24 h后,应用MTT(噻唑蓝)比色试验观察细胞增殖抑制率。结果橡胶防老剂4020在不同浓度下对HELF细胞的增殖抑制率与4010NA相比差异有统计学意义(P<0.01),且两种都表现出随着剂量增大而细胞毒性的作用增强。结论橡胶防老剂4020比4010 NA毒性低,应在我国轮胎制造行业积极推广应用。[Objective]To study the differences in toxicity and dose response trend of rubber antioxidant 4020 and 4010 NA on human embryonic lung fibroblast（HELF） cells. [Methods]HELF cells were exposed to different concentrations of 4020 and4010NA（3,6,9,12 μg/ml） for 24 hours. The cell proliferation inhibition rate was detected by MTT. [Results]There were statistically significant differences in the proliferation inhibition rates of HELF cells between 4020 and 4010 NA（P0.01）. And they all had dose response relationship. [Conclusion]Rubber antioxidant 4020 has lower toxicity as compared with 4010 NA,and it should be widely used in tire manufacturing industry in China.

关 键 词：4020 4010NA 人胚肺成纤维细胞(HELF) 增殖抑制率 

分 类 号：R114[医药卫生—卫生毒理学]