一株软腐病拮抗真菌的鉴定及生物学特性研究  

Identification and Biological Characterization of Antagonistic Fungi for Soft Rot Diseases

作　　者：魏蜜[1] 路露[1] 张伟[1] 傅本重[1] 王立华[1] 李国元[1] 

机构地区：[1]特色果蔬质量安全控制湖北省重点实验室,湖北工程学院生命科学技术学院,湖北孝感432000

出　　处：《北方园艺》2016年第15期100-104,共5页Northern Horticulture

基　　金：湖北省教育厅中青年人才资助项目(Q20152707);国家自然科学基金资助项目(31200488);特色果蔬质量安全控制湖北省重点实验室开放基金重点资助项目(2016K07,2014K02)

摘　　要：以从白菜软腐病根部土壤分离获得的1株能拮抗细菌性软腐病的拮抗真菌为试材,以平板对峙培养其抑菌圈达（37.7±2.2）mm,白菜离体叶片防效可达75%,依据形态特征结合rDNA-ITS序列分析,将获得的拮抗真菌鉴定为草茎点霉,并命名为Phoma herbarum F217-1,并研究了其生物学特性。结果表明：该菌株在马铃薯蔗糖培养基（PSA）上生长最佳,碳源和氮源以葡萄糖和硝酸钠较适宜,生长适温为28℃,致死温度为55℃,在pH 3~10条件下均生长良好,其最适pH 5~8,通气条件对草茎点霉菌丝生长均无明显影响,但光照24h条件下菌丝生长较差。A strain was isolated from soil of cabbage root which could antagonize soft rot diseases through bactericidal test and fermentation culture method.Its anti-bacterium-circle was（37.7±2.2）mm.Detached leaves experiment showed that its control efficienty was 75%.Following morphological characteristics,combining with the result of the rDNA-ITS sequence analysis,it was identified as Phoma herbarum and named Phoma herbarum F217-1.The results indicated that the F217-1grown well in PSA under 28 ℃,and the lethal temperature was 55 ℃.Although the mycelium could grow between pH 3-10,pH 5-8were appropriate.Glucose and NaNO3 were the suitable carbon and nitrogen resource,respectively.Ventilation had no significant effect on the growth of penicillium mycelium,but the 24 hours light was not benefit for the mycelia growth.

关 键 词：草茎点霉 鉴定 生物学特性 软腐病 拮抗作用 

分 类 号：S436.341.13[农业科学—农业昆虫与害虫防治]