鹿茸水提物减轻顺铂所致的小鼠肾损伤  

Protective effect of aqueous extract of velvet antler on cisplatin-induced nephrotoxicity in mice

作　　者：董思敏 王海璐[1] 王全凯[1] 张晶[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]长白山制药股份有限公司,吉林吉林132100

出　　处：《中国病理生理杂志》2016年第8期1466-1470,共5页Chinese Journal of Pathophysiology

基　　金：吉林省科技计划发展项目(No.20140204063YY;No.20130102058JC)

摘　　要：目的:探讨梅花鹿二杠茸和三岔茸水提物对顺铂(CDDP)所致小鼠肾损伤的影响。方法:采用灌胃给药方式,用顺铂(15 mg/kg)诱导小鼠肾损伤模型,测定小鼠肾脏指数(KI)、血清肌酐(SCr)、血尿素氮(BUN)、肾脏组织中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,并对肾脏组织进行HE染色,观察肾脏病理学变化,研究梅花鹿二杠茸和三岔茸的水提物各剂量对小鼠肾损伤的影响。结果:与顺铂组相比,各剂量鹿茸水提物可显著降低CDDP诱导肾损伤小鼠SCr、BUN水平及肾脏MDA含量,提高SOD和GSH-Px的活性(P<0.05);明显改善肾组织病理学形态,减轻CDDP对肾小管上皮细胞的损伤程度,且同等浓度下,与三岔茸相比,二杠茸水提物能更好地改善肾功能及减轻病理损伤。结论:鹿茸水提物减轻顺铂引起的小鼠肾损伤,其作用机制可能与鹿茸水提物增强小鼠肾脏组织的抗氧化能力有关。AIM： To study the protective effect of aqueous extract of 2-branched and 3-branched velvet antler on cisplatin（ CDDP）-induced nephrotoxicity in mice. METHODS：The mouse model of renal injury was induced by intragastric administration of CDDP at the dose of 15 mg / kg. After treatment,kidney index（ KI）,serum creatinine（ SCr）,blood urea nitrogen（ BUN）,the activity of superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px）,and the content of malondialdehyde（ MDA） in the kidney were determined. The renal pathological changes were observed with HE staining. RESULTS： Aqueous extract of velvet antler at the tested dose markedly decreased BUN,SCr and the content of MDA,and elevated the activity of SOD and GSH-Px in the mice pretreated with CDDP（ P 0. 05）. The pathological changes of the renal tissues were improved obviously,and the injury of the epithelial cells of renal tubules was mitigated. The effect of the aqueous extract of 2-branched velvet antler on renal function and cisplatin-induced nephrotoxicity was better than that of 3-branched one at the same concentration. CONCLUSION： The aqueous extract of 2-branched and 3-branched velvet antler has a certain protective effect on cisplatin-induced nephrotoxicity,which may be associated with increasing the anti-oxidative capability of mouse renal tissue.

关 键 词：鹿茸水提物 顺铂 肾毒性 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学] R363[医药卫生—中医学]