基质固相分散-共振光散射法测定蔬果中的甲基毒死蜱  

Determination of Chlorpyrifos-methyl in Vegetables and Fruit by Matrix Solid-Phase Dispersion Extraction and Resonance Light Scattering Method

作　　者：张传林[1] 胡庆红[1] 

机构地区：[1]遵义医学院药学院,贵州遵义563003

出　　处：《河南农业科学》2016年第8期81-85,共5页Journal of Henan Agricultural Sciences

基　　金：贵州省科学技术基金项目[黔科合J字LKZ(2011)44]

摘　　要：建立测定蔬果中甲基毒死蜱残留量的基质固相分散-共振光散射新方法,为蔬果中甲基毒死蜱的快速检测提供技术支持。以曲拉通X-100为稳定剂,甲基毒死蜱与灿烂甲酚蓝在p H值为10.00的Britton-Robinson缓冲液中相互作用,使体系共振光散射强度显著增强,并在360 nm、544 nm处出现特征散射峰。甲基毒死蜱质量浓度在0.1~5.0μg/m L与544 nm处的散射强度有良好的线性关系,其检出限为0.029μg/m L。各样品经基质固相分散法处理后,测得甲基毒死蜱的平均回收率为91.5%~96.8%。该方法快速、灵敏,可用于蔬果中甲基毒死蜱的检测。This study aimed to establish a new resonance light scattering( RLS) method for determination of chlorpyrifos-methyl in vegetables and fruit,providing technical support for rapid detection of chlorpyrifos-methyl in vegetables and fruit. In Britton-Robinson buffer(p H = 10. 00),with Triton X-100 as a stabilizer,the RLS intensity of chlorpyrifos-methyl greatly enhanced when brilliant cresyl blue was added.The scattering peaks were located at 360 nm and 544 nm. The RLS intensity was linear to the concentration of chlorpyrifors-methyl in the range of 0. 1—5. 0 μg / m L at 544 nm with a detection limit of 0. 029 μg / m L.Several samples were purified by matrix solid-phase dispersion( MSPD) and then determined. The average recoveries of chlorpyrifos-methyl in these samples ranged from 91. 5% to 96. 8%. This method was quick and sensitive for determination of chlorpyrifos-methyl residues in vegetables and fruit.

关 键 词：共振光散射 甲基毒死蜱 灿烂甲酚蓝 基质固相分散 

分 类 号：S481.8[农业科学—农药学]