黄连素促进肝癌细胞凋亡的氧化应激Wntβ-catenin信号通路机制研究  

The involvement of oxidative stress and Wnt/β-catenin signaling pathway in the apoptosis of hepatic carcinoma induced by berberine

作　　者：肖强[1] 毛盛名[1] 张斌[1] 吴君正 陈文哲[1] 

机构地区：[1]广州医科大学附属第六医院肝胆外科,广东清远511518

出　　处：《临床和实验医学杂志》2016年第16期1586-1589,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的对黄连素促进肝癌细胞凋亡的的氧化应激Wntβ-链蛋白(β-catenin)信号通路机制进行分析,为临床治疗提供借鉴。方法将生长状态良好的肝癌细胞随机分为4组:对照组、黄连素低剂量(1 m M)、中剂量(10m M)和高剂量(100 m M)组(n=8)。除对照组外,其余各组细胞给予对应剂量的黄连素共培养,分析各组细胞凋亡蛋白及氧化应激-Wntβ-catenin信号通路蛋白的表达。结果黄连素能剂量依赖性地增强肝癌细胞中Bax、Caspase3、Caspase9、P22、P67和Gp91蛋白表达而抑制Bcl2、Wnt、β-catenin、MMP2、MMP3、MMP9、TIMP1、TIMP2和TIMP3蛋白表达,差异有显著的统计学意义(P<0.05)。结论黄连素能有效促进肝癌细胞凋亡过程,且可能与其调控细胞内氧化应激及Wntβ-catenin信号通路机制有关。Objectiye The present research aimed to explore the involvement of oxidative stress and Wnt/ β - catenin signaling pathway in the apoptosis of hepatic carcinoma induced by berberine. Methods Hepatic carcinoma cell lines were divided into 4 groups,control,berberine at low（1 mM）,middle（10 mM）and high dose（100 mM）（n = 8）. The expression of oxidative stress and Wnt/ β - catenin signaling pathway re-lated proteins were assayed. Results The results showed that the expression of Bax,Caspase3,Caspase9,P22,P67 and Gp91 were increased greatly after the medication of berberine at low,middle and high dose（ P 〈 0. 05）. At the meaning time,the expression of Bcl - 2,Wnt,β -catenin,MMP2,MMP3,MMP9,TIMP1,TIMP2 and TIMP3 were decreased greatly after the medication of berberine at low,middle and high dose（ P 〈 0. 05）. Conclusion Berberine are effective on the inhibition of proliferation of hepatic carcinoma cell lines which maybe related with the regulation of oxidative stress and Wnt/ β - catenin signaling pathway.

关 键 词：肝癌 黄连素 氧化应激 细胞凋亡 Wntβ-catenin信号通路 

分 类 号：R285[医药卫生—中药学]